At5g63670 Antibody
Description
Gene and Protein Overview
The AT5G63670 gene encodes SPT4-2, a component of the SPT4/SPT5 complex. This complex interacts with RNAPII to facilitate transcription elongation, particularly in genomic regions with transcriptional stress . Key characteristics include:
Antibody Applications in Research
The At5g63670 Antibody is likely used to study SPT4-2 dynamics in Arabidopsis. While no direct experimental data are available, potential applications include:
Western Blotting and Immunoprecipitation
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Purpose: Detect SPT4-2 expression levels or post-translational modifications.
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Relevance: SPT4/SPT5 complexes are often regulated by phosphorylation in response to transcriptional stress .
Localization Studies
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Purpose: Map SPT4-2 distribution within plant cells (e.g., nucleus vs. cytoplasm).
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Method: Immunofluorescence or confocal microscopy.
Functional Knockdown Experiments
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Purpose: Assess the role of SPT4-2 in transcriptional fidelity or stress responses.
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Approach: Combine antibody detection with RNA-seq to identify genes affected by SPT4-2 depletion.
Research Gaps and Future Directions
| Research Area | Potential Focus |
|---|---|
| Transcriptional Stress | Investigate SPT4-2’s role in heat shock or DNA damage response in Arabidopsis. |
| Chromatin Interactions | Map SPT4-2 binding sites genome-wide using ChIP-seq. |
| Protein Interactions | Identify SPT4-2 binding partners (e.g., transcription factors, histone modifiers). |
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
