srb-3 Antibody

CD36/SR-B3 Antibody Overview

CD36, also known as scavenger receptor class B member 3 (SR-B3), is a transmembrane glycoprotein expressed on macrophages, endothelial cells, platelets, and adipocytes. It interacts with oxidized lipoproteins, fatty acids, and thrombospondin-1, influencing processes such as atherosclerosis, Alzheimer’s disease, and lipid transport . Antibodies targeting CD36/SR-B3 are used to study its function in these contexts.

Western Blot and Simple Western™ Analysis

• Human CD36 Detection: MAB19552 detects a specific band at 85–90 kDa in human placenta and platelet lysates under reducing conditions .

• Mouse CD36 Detection: AF2519 identifies bands at 125–138 kDa in adipose tissue and platelets, reflecting post-translational modifications .

Immunohistochemistry (IHC)

• Human Tissues: MAB19552 stains plasma membranes in heart tissue, highlighting CD36’s role in endothelial cell function .

• Mouse Tissues: AF2519 localizes CD36 to cytoplasm and plasma membranes in kidney and thymus sections, confirming its expression in immune cells .

Flow Cytometry

• Mouse Macrophages: AF2519 and MAB25191 enable detection of CD36 on J774A.1 macrophage cell lines, with distinct staining patterns .

Role in Lipid Metabolism

CD36 facilitates fatty acid uptake and oxidation, with antibodies like AF2519 used to study its regulation in hepatic fatty acid handling. For example:

• Fasting Studies: GADD45β-deficient mice show altered CD36/FATP2/FABP1 expression, linking CD36 to insulin signaling and lipid storage .

• Disease Models: CD36 knockout (KO) mice exhibit disrupted lipid metabolism, validated via Western blot quantification of related proteins (e.g., LC3B, S6K1) .

Inflammation and Atherosclerosis

• Oxidized LDL Clearance: CD36 mediates the uptake of oxidized low-density lipoproteins (oxLDLs), a key step in foam cell formation. Antibodies like MAB19552 are used to study this process in atherosclerotic plaques .

• Thrombospondin-1 Binding: CD36’s anti-angiogenic effects are critical in vascular diseases, with antibodies enabling functional studies in corneal neovascularization assays .

Alzheimer’s Disease and Neuroinflammation

• β-Amyloid Clearance: CD36 binds β-amyloid fibrils, and antibodies like AF2519 help assess its role in microglial phagocytosis and neurotoxicity .

Cross-Reactivity and Specificity

Challenges and Future Directions