sso7d Antibody
Description
Introduction to the Sso7d Antibody
The Sso7d antibody is a polyclonal immunoglobulin (IgG) raised against the recombinant DNA-binding protein Sso7d, a 7 kDa protein isolated from the hyperthermophilic archaeon Sulfolobus solfataricus. This antibody is widely used in molecular biology and biotechnology research for its ability to specifically recognize and bind to Sso7d and related proteins. Its applications span diagnostics, protein interaction studies, and the development of biotechnological tools .
Structure and Immunogen Details
The Sso7d antibody targets the Sso7d protein, a lysine-rich, sequence-independent DNA-binding protein that stabilizes genomic DNA under extreme conditions (high temperatures, acidity, and chemicals) . The antibody is generated by immunizing rabbits with recombinant Sso7d, purified via Protein A/G chromatography .
Sso7d Protein Features
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Molecular Weight: ~7 kDa (63 amino acids)
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Function: Binds dsDNA to condense and protect it in harsh environments .
3.1. ELISA and Western Blotting
The Sso7d antibody is validated for detecting Sso7d in enzyme-linked immunosorbent assays (ELISA) and Western blotting (WB). Its specificity ensures minimal cross-reactivity with non-target proteins, as demonstrated in studies involving co-immunoprecipitation .
3.2. Protein Interaction Studies
The antibody has been used to study Sso7d-mediated protein aggregation reversal and its role in stabilizing DNA polymerases in PCR reactions . For example, in co-immunoprecipitation experiments, it successfully pulled down Sso7d and associated proteins from archaeal cell extracts .
3.3. Biotechnological Development
Sso7d fusion proteins (e.g., Sso7d-Taq) are engineered to enhance PCR efficiency and inhibitor tolerance . The antibody aids in characterizing these fusions, ensuring proper expression and functionality .
5.1. Protein Aggregation Studies
The antibody has been used to demonstrate Sso7d’s role in reversing protein aggregates in archaeal cell extracts. Co-immunoprecipitation experiments showed that Sso7d associates with aggregated proteins, aiding their solubilization .
5.2. PCR Enhancement
In PCR applications, Sso7d fusion proteins (e.g., Sso7d-Taq) exhibit enhanced thermal stability and inhibitor tolerance. The antibody is used to confirm proper fusion protein expression and functionality .
5.3. Immunofluorescence
The antibody enables visualization of Sso7d localization in cells. For instance, it effectively labels Notch1-expressing cancer cells in immunofluorescent assays, with minimal background noise .
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
This sso7d antibody is generated through a process involving the immunization of a rabbit with recombinant Sulfolobus solfataricus DNA-binding protein sso7d (amino acids 2-64). This immunization triggers the production of IgG antibodies by the rabbit's B lymphocytes. The polyclonal sso7d antibody is subsequently purified using a protein A/G chromatography method. Researchers can utilize this sso7d antibody for the detection of Sulfolobus solfataricus sso7d protein in ELISA and Western blot assays.
The Sulfolobus solfataricus sso7d protein exhibits non-specific DNA binding through electrostatic interactions. Its primary role is to condense and stabilize DNA in extreme environments, safeguarding it from harsh conditions such as high temperatures, elevated salinity, and extreme pH. In vitro experiments have demonstrated that sso7d facilitates the annealing of complementary DNA strands, induces negative supercoiling, and acts as a chaperone in the disassembly and renaturation of protein aggregates, all in an ATP hydrolysis-dependent manner.
Target Background
KEGG: sso:SSO10610
STRING: 273057.SSO10610
