Stat5b Antibody
Description
Structure and Epitope
The Stat5b Antibody is typically a mouse monoclonal IgG1 antibody. For instance, the Santa Cruz Biotechnology product (Catalog #sc-1656) targets an epitope mapping between amino acids 750–779 at the C-terminus of mouse-origin Stat5b, ensuring cross-reactivity with human, rat, and other species like equine and porcine . This specificity allows researchers to study STAT5B in both murine models and human tissues.
In contrast, the R&D Systems antibody (Catalog #MAB1584) employs a distinct epitope, validated through Western blot and flow cytometry experiments in human cell lines (e.g., HeLa, Daudi) and knockout cell lines to confirm specificity .
Applications
The Stat5b Antibody is versatile, supporting multiple experimental techniques:
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Western Blot (WB): Detects STAT5B at approximately 90–91 kDa under reducing conditions. Knockout cell line validation (e.g., HeLa STAT5b KO) ensures no cross-reactivity with STAT5A .
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Immunoprecipitation (IP): Used to isolate STAT5B complexes for downstream analysis, as demonstrated in HAP1 human cells .
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Immunofluorescence (IF): Localizes STAT5B in cellular compartments, aiding studies on nuclear translocation post-cytokine stimulation .
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Flow Cytometry: The R&D Systems antibody (MAB1584) enables detection of STAT5B in Jurkat T cells, distinguishing it from isotype controls .
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ELISA: The Santa Cruz antibody facilitates quantification of STAT5B in lysates or sera .
Role in Hematological Malignancies
STAT5B is implicated in BCR/ABL-positive leukemias, where its activation suppresses interferon (IFN)-α/γ signaling, promoting tumor growth. Studies using Stat5b-deficient mice revealed impaired leukemic colony formation, underscoring STAT5B’s role in oncogenesis . Antibodies like sc-1656 (Santa Cruz) have been employed to confirm STAT5B activation in patient-derived leukemia cells.
Gain-of-Function Mutations
Gain-of-function mutations in STAT5B have been linked to early-onset immune disorders, including atopic dermatitis and mast cell-driven inflammation. Functional assays using patient-derived T cells demonstrated hyperresponsiveness to cytokines, with the Stat5b Antibody validating elevated protein levels .
Tissue-Specific Expression
STAT5B exhibits sexual dimorphism in liver gene expression, regulating metabolism and detoxification. The antibody has been used to study gender-specific STAT5B activity in rodent models .
Western Blot Validation
| Cell Line | Detection | Band Size | Conditions |
|---|---|---|---|
| HeLa | STAT5B | 90 kDa | Reducing |
| Daudi | STAT5B | 90 kDa | Reducing |
| STAT5b KO HeLa | None | — | Reducing |
Product Specs
Target Background
- STAT5 dimers are essential for NK cell development, while STAT5 tetramers mediate NK cell maturation and the expression of genes associated with maturation. This was demonstrated in studies using tetramer-deficient double knockin mice where NK cell numbers were decreased. PMID: 29105654
- Research has shown that RGS4, a regulator of G protein signaling, is involved in opioid-dependent neuronal differentiation and neurite outgrowth. This suggests that RGS4 influences STAT5B-directed responses through a non-canonical signaling pathway. PMID: 28219718
- STAT5B is a critical regulator of receptor-induced cell death (RICD) in memory T cells in mice. PMID: 29187589
- Research has indicated that an active form of Stat5b restored tolerogenic functions in dendritic cells (DCs). These DCs then re-educated regulatory T cells (Tregs) to establish and sustain long-term protective immune responses against diabetes in NOD mice. PMID: 27634616
- Studies have shown that 89% of signal transducer and activator of transcription 5 (Stat5b)-CA mice, where the T2/Onc transposon had been mobilized, died of progenitor B-cell acute lymphoblastic leukemia (B-ALL) by 3 months of age. PMID: 26500062
- STAT5B and MOF act as negative regulators in adipogenesis. PMID: 26388045
- Liver STAT5b activation, associated with masculinization, occurs during puberty. Conversely, suppression and feminization occur during aging and in mutant mice with defects in growth hormone signaling. PMID: 26959975
- Loss of STAT5b did not have any effect on either sex. PMID: 24838184
- Increased expression of STAT5b along with PDGFB led to increased expression of downstream STAT5b targets. PMID: 25302990
- The non-obese-diabetic genetic background is critical for Stat5b-mediated lymphomagenesis through the regulation of Stat5 hyperactivation. PMID: 23457589
- Stat5b provides protection against diabetes in NOD mice by regulating the numbers and function of various immune cell types, particularly by upregulating CD4(+)CD25(+) regulatory T cells. PMID: 22789848
- STAT5 serves as a negative regulator of follicular helper (TFH) CD4+ T cell differentiation, primarily through the regulation of the transcription factor Blimp-1. PMID: 22271576
- CPEB3, a cytoplasmic polyadenylation element binding protein, exhibits a novel function in the nucleus. It suppresses Stat5b-dependent EGFR gene transcription. PMID: 20639532
- In Treg-depleted Stat5b-CA transgenic (TG) versus wild-type (WT) CD4(+)CD25(-) Teffs, GVHD lethality was reduced, which was attributed to decreased Teff proinflammatory responses and increased Th2 anti-inflammatory cytokine responses. PMID: 20442366
- Growth enhancement in suppressor of cytokine signaling 2 (SOCS-2)-deficient mice is dependent on signal transducer and activator of transcription 5b (STAT5b). PMID: 12040024
- Stat5 regulates the expression of peroxisome proliferator-activated receptor-gamma, highlighting a novel role of Stat5 in adipocyte differentiation. PMID: 12089351
- While Stat5b-/- knockout mice exhibit decreased numbers of CD8+ memory cells, transgenic Stat5b expression significantly increases CD8+ memory T cell numbers, indicating its critical role in regulating the homeostasis of this cell population. PMID: 12496402
- STAT 5 proteins are not activated by physiological levels of insulin in adipose tissue. PMID: 12604355
- A subset of STAT5B-deficient mice display autoimmune pathology similar to mice lacking IL-2 or its receptor components, characterized by lymphocytic infiltration of multiple organs, including the bone marrow. PMID: 14607901
- The Stat5b pathway is a key molecular defect in NOD mice. PMID: 14701862
- Prolactin activation of Stat5b is crucial in suppressing 20alpha-HSD gene expression, thereby maintaining progesterone levels necessary for sustaining pregnancy. PMID: 15471942
- STAT5b plays a critical role during steady-state hematopoietic stem cell engraftment, and a chromosome 7 modifier locus regulates this activity. PMID: 15498858
- Research suggests that STAT5 proteins are involved in the feedback regulation of somatostatin neurons by GH, and these neurons may respond to patterned GH secretion to reinforce sexual dimorphism in the GH axis. PMID: 15796771
- Studies suggest the potential involvement of STAT5b in Lck-mediated cellular transformation. PMID: 16446405
- Several female-predominant repressors are elevated in STAT5b-deficient males, potentially contributing to the significant loss of male gene expression observed in the absence of STAT5b. PMID: 16469768
- The cleaved cytosolic fragment of ICA512 enhances the transcription of secretory granule genes (including its own gene) by binding to tyrosine phosphorylated signal transducers and activators of transcription (STAT) 5 and preventing its dephosphorylation. PMID: 16622421
- STAT5B protein plays a vital role in preimplantation development by mediating signals from cytokines. PMID: 16775227
- Transgenic mice expressing a constitutively active mutant of STAT5b (CASTAT5) under control of the rat insulin 1 promoter exhibited increased glucose tolerance and reduced hyperleptinemia compared to wild-type mice when subjected to a high-fat diet. PMID: 17003334
- Therapeutic activation of the GH:STAT5b axis presents a novel target for restoring both normal anabolic metabolism and mucosal tolerance in Crohn's disease (CD). PMID: 17148664
- The significant loss of liver sexual dimorphism in hepatocyte STAT5ab-deficient mice can primarily be attributed to the loss of STAT5b. PMID: 17317776
- A defective JAK-Stat5B pathway in NOD mice, along with other defective factors, is associated with the pathogenesis of autoimmune type 1 diabetes in NOD mice. PMID: 17382905
- The transcription factor Stat5b is not required for islet development but modulates beta-cell physiology upon aging. PMID: 17599554
- Stat5b directly controls IL-7-mediated early B cell development but is not required for B cell maturation and Ig production. PMID: 17617599
- Alterations in T-cell development at the double-negative/double-positive (DN/DP) stages cooperate with cytokine-mediated pathways in immature thymocytes to give rise to lymphoblastic T-cell lymphomas in Stat5b TG mice. PMID: 17890450
- Murine OSMR initiates STAT5 activation directly via the receptor-bound Janus kinases. Interestingly, the murine receptor preferentially recruits JAK2, while the human receptor seems to have a higher affinity for JAK1. PMID: 18430728
- Enhanced SULT1E1 activity may play a role in inhibiting GH-stimulated STAT5b phosphorylation and IGF-1 synthesis through the sulfation and inactivation of E2. PMID: 18831980
- STAT5b signaling is critical for leukemia stem cell self-renewal in MN1- and HOXA9-expressing leukemias. PMID: 19667399
