STK17B Human
Description
Molecular Structure and Expression
STK17B is encoded by the STK17B gene located on chromosome 2 (NCBI Gene ID: 9262) . The crystal structure of human STK17B (PDB ID: 3LM0) reveals a conserved kinase domain with a resolution of 2.35 Å, critical for its enzymatic activity (Fig. 1) .
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Comprises 395 amino acids (44.7 kDa molecular mass).
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Contains a His-tag for recombinant purification.
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Interacts with ligands such as pyrimidine derivatives and chloride ions.
STK17B is expressed in multiple tissues, with high levels observed in the placenta, lung, and pancreas, and lower expression in the heart, brain, and liver . In the cerebellum, it regulates dendritic development in Purkinje cells via protein kinase C (PKC) signaling .
Apoptosis Regulation
STK17B acts as a positive regulator of apoptosis in specific contexts:
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Promotes apoptosis in islet β-cells and acute myeloid leukemia cells .
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Binds CHP1 (calcineurin B homologous protein 1), triggering its nuclear translocation .
Cancer Biology
STK17B exhibits dual roles in cancer (Table 1):
In HCC, STK17B overexpression drives metastasis by:
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Inducing epithelial-mesenchymal transition (EMT): Downregulates E-cadherin and upregulates N-cadherin/vimentin .
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Activating AKT/GSK-3β/Snail signaling: Enhances phosphorylation of AKT and GSK-3β, stabilizing Snail to promote EMT (Fig. 2) .
Immune System Modulation
STK17B is critical in T-cell receptor (TCR) signaling and immune tolerance:
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T-cell activation: Inhibition lowers TCR activation thresholds, enhancing IL-2 and IFN-γ secretion .
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Therapeutic potential:
Recombinant STK17B and Research Tools
The recombinant human STK17B protein (Prospec Bio, Cat# PKA-050) is produced in E. coli with the following specifications :
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Purity: >90% (SDS-PAGE).
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Formulation: 0.5 mg/mL in Tris-HCl buffer (pH 8.0) with 10% glycerol.
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Applications: Kinase assays, apoptosis studies, and structural biology.
Clinical and Therapeutic Implications
Product Specs
MGSSHHHHHH SSGLVPRGSH MGSMSRRRFD CRSISGLLTT TPQIPIKMEN FNNFYILTSK ELGRGKFAVV RQCISKSTGQ EYAAKFLKKR RRGQDCRAEI LHEIAVLELA KSCPRVINLH EVYENTSEII LILEYAAGGE IFSLCLPELA EMVSENDVIR LIKQILEGVY YLHQNNIVHL DLKPQNILLS SIYPLGDIKI VDFGMSRKIG HACELREIMG TPEYLAPEIL NYDPITTATD MWNIGIIAYM LLTHTSPFVG EDNQETYLNI SQVNVDYSEE TFSSVSQLAT DFIQSLLVKN PEKRPTAEIC LSHSWLQQWD FENLFHPEET SSSSQTQDHS VRSSEDKTSK SSCNGTCGDR EDKENIPEDS SMVSKRFRFD DSLPNPHELV SDLLC.
Product Science Overview
The STK17B gene is located on chromosome 2 and encodes a protein consisting of 372 amino acids . The protein has several key functional domains, including ATP-binding sites and an active site essential for its kinase activity . The enzyme’s catalytic activity involves transferring a phosphate group from ATP to serine or threonine residues on substrate proteins .
STK17B is primarily involved in the regulation of apoptosis, a programmed cell death process crucial for maintaining cellular homeostasis . It acts as a positive regulator of apoptosis by phosphorylating myosin light chains, which are involved in the contraction of the actin cytoskeleton . Additionally, STK17B is implicated in intracellular signal transduction and protein phosphorylation, further highlighting its role in cellular regulation .
STK17B is associated with several biological pathways, including:
These pathways underscore the kinase’s involvement in both sensory perception and cellular degradation processes. Moreover, STK17B interacts with various proteins and participates in complex signaling networks that regulate cell survival and death .
Mutations or dysregulation of the STK17B gene have been linked to certain diseases, such as exudative glomerulonephritis . Understanding the function and regulation of STK17B can provide insights into the molecular mechanisms underlying these conditions and potentially lead to the development of targeted therapies.
Human recombinant STK17B is widely used in research to study its biochemical properties and role in cellular processes. Recombinant proteins are produced through genetic engineering techniques, allowing scientists to investigate the kinase’s function in vitro and in vivo. These studies contribute to our understanding of STK17B’s role in health and disease and may pave the way for novel therapeutic approaches.
