At4g00130 Antibody

Gene Background and Antibody Target

The AT4G00130 gene in Arabidopsis thaliana encodes a protein with potential roles in DNA-binding or chromatin-related processes. While the precise molecular function remains under investigation, its involvement in epigenetic studies is suggested by its association with histone modifications and transcriptional regulation .

Antibody Applications

The At4g00130 Antibody is primarily employed in:

• Chromatin Immunoprecipitation (ChIP): To study DNA-protein interactions and identify binding sites of the AT4G00130 protein in plant genomes .

• Molecular Cloning Experiments: As part of Gateway cloning workflows for gene expression studies in Arabidopsis .

• Western Blotting (WB): For detecting endogenous AT4G00130 protein levels in plant tissues.

Epigenetic Studies

In a study analyzing histone modifications, the At4g00130 Antibody demonstrated a relative enrichment of 2.452 (p-value: 6.56E-08) for DNA-binding regions, indicating its utility in mapping protein-DNA interactions . This suggests the AT4G00130 protein may regulate chromatin accessibility or transcriptional activation.

Cloning and Gene Expression

The gene was cloned into effector vectors (e.g., pDB-HT-GAL4-HFC) using Gateway cloning, enabling functional analysis in Arabidopsis. This approach highlights its role in transcriptional regulation assays .

Comparison with Related Antibodies

Below is a comparison of the At4g00130 Antibody with other plant-specific antibodies from the same supplier:

Data compiled from Cusabio product listings .

Methodological Considerations

• ChIP Protocol: Requires optimization of crosslinking and sonication conditions to ensure specific DNA-protein complexes are captured .

• Cross-Reactivity: No reported cross-reactivity with non-Arabidopsis species, making it suitable for targeted studies .