At4g27130 Antibody
Description
Target Identification: At4g27130 Gene
The At4g27130 gene encodes the δ subunit of the eIF2B complex, a critical regulator of mRNA translation. This subunit facilitates the recycling of eIF2·GDP to eIF2·GTP, a rate-limiting step in translation initiation .
| Gene Attribute | Details |
|---|---|
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Protein Product | eIF2Bδ subunit |
| Uniprot ID | P41568 |
| Molecular Function | Guanine nucleotide exchange factor activity for eIF2 |
| Biological Process | Regulation of translational initiation in response to stress |
3.1. Translation Regulation Studies
The eIF2B complex is pivotal under stress conditions such as heat, salinity, or nutrient deprivation. The At4g27130 antibody enables researchers to:
-
Investigate post-translational modifications affecting translation initiation.
3.2. Genetic Knockout Validation
This antibody is used to confirm the absence of eIF2Bδ in knockout Arabidopsis lines, ensuring genetic modifications are effective .
3.3. Subcellular Localization
Immunohistochemistry assays using this antibody help map eIF2Bδ distribution in plant cells, particularly in cytoplasmic foci under stress .
Key Research Findings
-
Stress-Induced Expression: Transcriptomic data indicate At4g27130 is downregulated under hypoxia (log2 fold change = -0.39, p = 0.037), suggesting its role in adaptive translation during stress .
-
Conservation Across Species: Homologs of eIF2Bδ in yeast and mammals share structural motifs critical for guanine nucleotide exchange, highlighting evolutionary conservation .
Future Directions
Further studies could explore:
-
Interactions between eIF2Bδ and other translation initiation factors.
-
The impact of phosphorylation on eIF2Bδ activity during abiotic stress.
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
