sun-1 Antibody
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- The coiled-coil domains of SUN-1 are essential for protein oligomerization and retention within the nuclear envelope, particularly during later stages of female gametogenesis. PMID: 27098914
- POT-1 contributes to anchoring telomeres to the nuclear periphery via interactions with SUN-1. PMID: 24297748
- Phosphorylation of SUN-1 appears to be critical in delaying meiosis in response to disrupted synapsis, suggesting a potential role in a checkpoint mechanism monitoring the establishment of obligate crossover events. PMID: 23505384
- The dynamics of matefin/SUN-1 aggregates remain consistent throughout leptonene/zygotene, despite the progression of chromosome pairing. PMID: 21124819
- [REVIEW] This study examines the functions of KASH/SUN protein pairs across various spatial and temporal stages of C. elegans development. PMID: 20108325
- Research demonstrates that ZYG-12 is immobile at the outer nuclear membrane, and that SUN-1 is sufficient for ZYG-12 localization within cells. PMID: 19759181
- Findings suggest that alterations in the properties of the nuclear envelope occur during the timeframe when homologous chromosomes are sorted and matefin/SUN-1 aggregates form, potentially influencing the movement, pairing, and recombination of chromosomes. PMID: 19913286
Q&A
How do I validate SUN1 antibody specificity for nuclear envelope proteins in mammalian cells?
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Methodological approach:
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Perform Western blot using lysates from SUN1-knockout cell lines (e.g., CRISPR-edited HT1080 cells) alongside wild-type controls. Expect bands at ~90-102 kDa depending on isoform expression .
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Combine with immunofluorescence to verify nuclear envelope localization, using co-staining with lamin B1 as a spatial reference .
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Validate via siRNA knockdown followed by quantitative PCR to confirm reduced SUN1 mRNA levels correlate with diminished antibody signal .
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What experimental strategies address contradictory molecular weight observations (90 vs. 100 kDa) in Western blot?
How to design SUN1 functional studies in nuclear-cytoskeletal coupling?
Advanced protocol:
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Live-cell imaging: Transfect SUN1-GFP constructs with KASH5-mCherry to visualize LINC complex dynamics during nucleokinesis .
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Biomechanical assays: Use atomic force microscopy to measure nuclear stiffness in SUN1-depleted cells vs. controls .
What controls are essential when studying SUN1 in HIV-1 restriction mechanisms?
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Viral controls: Include HIV-1-GFP with capsid mutation G208R (resistant to SUN1-mediated restriction) .
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Cellular controls:
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Binding assays: Use in vitro assembled HIV-1 CA-NC complexes to test direct SUN1-capsid interaction .
How to resolve tissue-specific SUN1 expression patterns in IHC?
What advanced techniques characterize SUN1's role in DNA repair?
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γH2AX co-staining: Quantify DNA damage foci in SUN1-depleted cells after ionizing radiation .
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Comet assay: Compare DNA migration patterns between wild-type and SUN1-knockout cells .
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CHIP-seq: Map SUN1 binding sites near fragile genomic regions using ab124770 for chromatin immunoprecipitation .
