SUN2 Antibody, HRP conjugated
Description
Definition and Mechanism
The HRP-conjugated SUN2 antibody is a recombinant rabbit monoclonal antibody directly linked to HRP, eliminating the need for secondary antibodies in detection workflows. It binds specifically to SUN2, a core component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex, which bridges the nuclear lamina and cytoskeleton to regulate nuclear positioning and mechanotransduction .
Key Features:
-
Conjugate: Horseradish peroxidase (HRP) enables chemiluminescent or colorimetric signal amplification .
-
Target: SUN2 (UniProt ID: Q9UH99), with an observed molecular weight of ~80 kDa .
-
Applications: WB, IHC, immunofluorescence (IF/ICC), and flow cytometry .
Applications and Validation
HRP-conjugated SUN2 antibodies are validated across diverse experimental models:
Table 1: Key Applications and Performance
Research Findings
HRP-conjugated SUN2 antibodies have facilitated discoveries in virology, oncology, and neurobiology:
A. Viral Pathogenesis
-
HIV-1 Latency: SUN2 knockdown in CD4+ T cells reactivates HIV-1 transcription by converting chromatin to an active state, suggesting SUN2’s role in maintaining viral latency .
-
HSV-1 Propagation: SUN2 depletion in 143B cells increases HSV-1 yields by 6-fold, implicating SUN2 in restricting viral replication .
-
Zika Virus Replication: SUN2 promotes viral RNA replication and replication organelle formation, independent of viral entry .
B. Cancer and Cellular Dynamics
-
Nuclear Migration: SUN2 is essential for interkinetic nuclear migration in neuronal and glial cells, linking nuclear positioning to cytoskeletal dynamics .
-
Chromatin Remodeling: SUN2 binds lamin A/C to maintain repressive chromatin states, as shown in HIV-1 latency studies .
Technical Considerations
-
Signal Amplification: HRP catalyzes substrate oxidation (e.g., TMB for colorimetry or luminol for chemiluminescence), enabling high sensitivity .
-
Validation Controls: Include knockout cell lines (e.g., SUN2KO Huh7 cells ) and isotype-matched antibodies to confirm specificity .
-
Storage: Stable at -20°C; avoid freeze-thaw cycles to preserve HRP activity .
Case Study: SUN2 in Viral Research
A 2023 study utilized SUN2 antibodies to demonstrate that disulfide bonds in SUN2 regulate its interaction with KASH-domain proteins (e.g., NESPRIN3). By substituting cysteine residues (C577A, C719A), researchers showed that SUN2’s conformational changes influence viral replication efficiency .
Future Directions
HRP-conjugated SUN2 antibodies are pivotal for exploring:
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Research findings demonstrate that SUN2 is essential for optimal activation and proliferation of primary CD4 T cells. The disruption of these processes likely explains the contribution of endogenous SUN2 to HIV infection in primary lymphocytes. Notably, CypA is not required for the decreased infection observed in SUN2-silenced cells. PMID: 28077629
- SUN2 exerts tumor suppressor functions by suppressing the Warburg effect in lung cancer. PMID: 26658802
- Overexpression of SUN2 disrupts both nuclear shape and the early stages of HIV infection. PMID: 26865710
- SUN2 acts as a tumor suppressor, mediating the functions of miR-221 and miR-222 in central nervous system embryonal tumors. PMID: 24832085
- The crystal structure of the SUN2 protein SUN domain has been determined. PMID: 22170055
- Data indicate that expression of lamin A, but not lamin C, partially restored the nuclear envelope localization of SUN2. PMID: 21655223
- Research suggests that SUN2 is the first mammalian inner nuclear membrane (INM) protein reliant on a functional nuclear localization signal, a Golgi retrieval signal, and a perinuclear domain to mediate targeting to the INM. PMID: 20551905
