SVR1 Antibody
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Product Specs
Buffer
**Preservative:** 0.03% Proclin 300
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Made-to-order (14-16 weeks)
Synonyms
SVR1 antibody; PDE328 antibody; At2g39140 antibody; Putative ribosomal large subunit pseudouridine synthase SVR1 antibody; chloroplastic antibody; EC 5.4.99.- antibody; Protein PIGMENT DEFECTIVE 328 antibody; Protein SUPPRESSOR OF VARIEGATION 1 antibody
Target Names
SVR1
Uniprot No.
Target Background
Function
SVR1 is a protein responsible for the synthesis of pseudouridine in chloroplastic 23S ribosomal RNA. It plays a crucial role in normal chloroplast rRNA processing and translation. SVR1 is essential for proper chloroplast development and maintenance. Additionally, it may have functions in other plastids, such as root amyloplasts.
Gene References Into Functions
- Research indicates that SVR1/At2g39140 suppresses var2-mediated leaf variegation. It not only participates in uridine isomerization but also its physical presence is necessary for proper chloroplast rRNA processing. PMID: 18599582
Database Links
Protein Families
Pseudouridine synthase RsuA family
Subcellular Location
Plastid, chloroplast.
Tissue Specificity
Highly expressed in young seedlings. Expressed in roots, rosette leaves, cauline leaves, stems and flowers.
Q&A
Advanced Research Questions
How to resolve contradictory results in SVR1 binding assays?
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Analyze antibody-epitope interactions through HDX-MS or cryo-EM
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Test binding kinetics under physiological conditions (BLI/SPR preferred over ELISA)
What computational approaches enhance antibody design for SVR1?
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Use Rosetta-based CDR optimization (achieved 20-fold affinity improvement in SARS-CoV-2 RBD antibodies)
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Validate with molecular dynamics simulations (>100ns production runs)
Methodological Challenges
How to optimize NGS analysis for SVR1 antibody repertoires?
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Process raw sequences with adaptive clustering thresholds:
Parameter Recommended Value Rationale CDR3 Length ±3 aa from germline Maintain structural flexibility V-J Alignment >85% identity Preserve framework integrity
Validation Standards
What metrics define high-performing SVR1 antibodies?
| Performance Tier | Binding Affinity | Signal:Noise Ratio | Cross-Reactivity |
|---|---|---|---|
| Excellent | KD < 10 nM | ≥5:1 | None detected |
| Acceptable | KD 10-100 nM | ≥3:1 | <5% homologs |
| Poor | KD > 100 nM | <3:1 | Uncontrolled |
| Data synthesis from |
Key Limitations in Current Research:
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No peer-reviewed validation data exists for SVR1-specific reagents in the provided literature
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Epitope conservation analysis unavailable without structural data
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Functional assays (neutralization/blocking) remain theoretical for this target
Researchers should:
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