SYCE1 Antibody

Biological Role of SYCE1

SYCE1 forms the structural core of the SC, a tripartite protein scaffold essential for homologous chromosome pairing during meiosis. Its N-terminal region (amino acids 25–179) adopts an anti-parallel coiled-coil structure, enabling interactions with other SC components like SIX6OS1 . Mutations in SYCE1, such as c.375-2A>G (associated with non-obstructive azoospermia) and c.613C>T (linked to premature ovarian failure), disrupt SC assembly and meiotic progression .

Western Blot Analysis

The antibody detects SYCE1 expression in lysates of human, mouse, and rat cells. For example, in a study of a novel SYCE1 mutation (c.689_690del; p.F230fs), Western blot revealed reduced SYCE1 expression in patient blood cells and transfected 293T cells . The protocol involves lysing cells in RIPA buffer, denaturing proteins with SDS, and detecting the target using HRP-conjugated secondary antibodies .

Immunohistochemistry (IHC)

IHC protocols validate SYCE1 localization in tissues. In the same mutation study, wild-type SYCE1 clustered in the nucleus, while mutant SYCE1 exhibited cytoplasmic diffusion, highlighting the antibody’s utility in studying protein mislocalization .

Male Infertility

Male SYCE1-null mice exhibit arrested spermatogenesis and accelerated apoptosis of primary spermatocytes, underscoring the antibody’s role in studying meiotic defects .

Female Infertility

Mutations like c.613C>T (POF) truncate SYCE1, disrupting its second binding interface with SIX6OS1. This impairs synapsis and chromosome segregation, leading to ovarian failure .

Protocols and Resources

The manufacturer provides downloadable protocols for WB, IHC, IF, and IP. Standard protocols for these techniques are also available .