At4g33760 Antibody

Subcellular Localization and Targeting Mechanisms

Studies employing in vitro import assays and fluorescence colocalization revealed:

Key Findings:

• Mitochondrial Import: Strong, consistent signals observed across experiments .

• Chloroplast Import: Weaker fluorescence but validated via in vitro assays .

• Targeting Signals: Predicted by predotar (Mit: 0.50, Chloro: 0.01) and TargetP (Mit: 0.32, Chloro: 0.36) .

The N-terminal sequence (residues 1–80) contains overlapping mitochondrial and chloroplast transit peptides, enabling dual targeting .

Functional Role in Organellar Translation

At4g33760 AspRS is essential for protein synthesis in both mitochondria and chloroplasts:

• Catalyzes tRNA-Asp aminoacylation, ensuring accurate translation .

• Phylogenetically related to cyanobacterial AspRS, suggesting endosymbiotic origin .

• Dual targeting compensates for the absence of separate organellar AspRS isoforms in plants .

Research Applications of At4g33760 Antibody

Commercial antibodies (e.g., CSB-PA599827XA01DOA) enable specific detection in:

• Western Blot: Identifies ~60 kDa protein in mitochondrial/chloroplast lysates .

• Immunofluorescence: Visualizes punctate organellar signals in plant tissues .

• Subcellular Fractionation Studies: Validates dual localization .

Research Implications

Dual-targeted aaRSs like At4g33760 highlight evolutionary adaptations in plant organellar communication. This antibody facilitates studies on:

• Organellar translation fidelity under stress .

• Evolutionary links between cyanobacteria and chloroplasts .

• Cross-compartment tRNA synthetase redundancy .