TAS2R13 Antibody

Introduction to TAS2R13 Antibody

TAS2R13 antibodies target the taste receptor type 2 member 13 (TAS2R13), a protein encoded by the TAS2R13 gene located on chromosome 12p13 . This receptor is part of the bitter taste perception system and is linked to gustducin-mediated signaling pathways that influence responses to bitter compounds . Antibodies against TAS2R13 are critical for studying its role in taste biology, gastrointestinal chemosensing, and potential therapeutic applications .

Functional Insights

• Bitter Taste Perception: TAS2R13 activation triggers signaling via α-gustducin, phospholipase C-β2 (PLC-β2), and TRPM5 channels, facilitating calcium signaling in response to bitter tastants .

• Extraoral Roles: TAS2R13 is implicated in monitoring gastrointestinal content composition, suggesting roles in gut-brain communication and metabolic regulation .

Experimental Validation

• Western Blot: Antibody NBP3-09892 detects TAS2R13 at ~33 kDa in human HCT15 cell lysates .

• Immunohistochemistry: STJ95870 localizes TAS2R13 in human tissue sections, confirming membrane-associated expression .

Performance Metrics

Applications in Research

• Western Blot (WB): Used to confirm TAS2R13 expression in cell lysates (e.g., HCT15 colorectal carcinoma cells) .

• Immunohistochemistry (IHC): Maps receptor distribution in taste buds and gastrointestinal epithelia .

• ELISA: Quantifies TAS2R13 levels in biological samples .

Validation and Quality Control

• Specificity: Antibodies are validated using knockout cell lines or competitive peptide assays to ensure minimal cross-reactivity .

• Citations: ABIN1536831 and NBP3-09892 cite peer-reviewed studies in their validation data .