TBC1D25 Antibody, Biotin conjugated

Molecular and Functional Overview

TBC1D25 Protein:

• Function: Acts as a GTPase-activating protein (GAP) specific for RAB33B, regulating autophagosome-lysosome fusion .

• Structure: 688 amino acids, observed molecular weight ~75–80 kDa .

• UniProt ID: Q3MII6 .

Biotin Conjugation:

• Purpose: Enhances detection via high-affinity binding to streptavidin ($K_D = 10^{-14}–10^{-15}$), surpassing traditional antibody-antigen interactions ($K_D = 10^{-7}–10^{-11}$) .

• Advantages: Small biotin size (240 Da) minimizes steric hindrance, enabling efficient labeling without disrupting antibody specificity .

Applications and Protocols

Research Findings

• Autophagy Regulation: TBC1D25 knockdown disrupts autophagosome maturation, linking it to neurodegenerative and metabolic diseases .

• Cardioprotective Role: In ischemia-reperfusion injury models, TBC1D25 suppresses TAK1-JNK/p38 MAPK signaling, reducing myocardial apoptosis and inflammation .

• Technical Validation: Biotin-conjugated antibodies show 3-fold higher enrichment efficiency compared to anti-biotin antibodies in peptide identification assays .

Technical Considerations

• Storage: Stable at -20°C for >1 year; avoid freeze-thaw cycles .

• Hazard Notes: Contains Proclin 300, a preservative requiring careful handling .

• Controls: Include biotin-free controls to rule out nonspecific streptavidin binding .