TCF15 Antibody

Overview of TCF15 Antibody

TCF15 antibodies are immunological reagents designed to detect and quantify the TCF15 protein, a basic helix-loop-helix (bHLH) transcription factor involved in cellular differentiation, epithelialization, and stem cell regulation . These antibodies are widely used in techniques such as Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF) to investigate TCF15’s roles in development and disease.

Research Applications

TCF15 antibodies enable critical insights into:

• Pluripotency and Differentiation: TCF15 marks differentiation-primed subpopulations in embryonic stem cells (ESCs) and regulates exit from naive pluripotency .

• Hematopoietic Stem Cell (HSC) Quiescence: TCF15 maintains HSC quiescence and long-term self-renewal, as shown in in vivo CRISPR screens .

• Epithelialization: TCF15 coordinates epithelial morphogenesis by upregulating integrin α3β1, a laminin receptor .

Role in Stem Cell Biology

• Priming Differentiation: TCF15 expression in ESCs correlates with reduced Nanog and Klf4 levels, marking a transition from naive to primed pluripotency .

• HSC Regulation:

  • TCF15 knockout reduces long-term HSC engraftment capacity by 20.8-fold .

  • Overexpression enforces quiescence, enriching LT-HSCs while depleting progenitors .

Mechanisms of Action

• Gene Regulation: TCF15 modulates genes like Cdkn1c, Mcl1, and Gata2 to maintain stem cell quiescence .

• Interaction with Id Proteins: Id proteins inhibit TCF15 activity, delaying differentiation until downregulated .

Developmental Roles

• Somitogenesis: TCF15 is essential for epithelialization of somites during embryogenesis .

• Laminin Receptor Upregulation: TCF15 drives integrin α3 expression, enabling epithelial morphogenesis .

Validation and Quality Control

• Boster Bio: Validated in WB (1:500–2,000 dilution) and ELISA (1:5,000–20,000) .

• Novus Biologicals: Demonstrated nuclear speckle localization in HEK 293 cells via IF .