TCF19 Antibody, FITC conjugated

Introduction to TCF19 Antibody, FITC Conjugated

TCF19 (Transcription Factor 19) is a nuclear protein implicated in cell cycle regulation, DNA damage response, and cancer progression . The FITC (Fluorescein Isothiocyanate)-conjugated TCF19 antibody is a fluorescently labeled reagent designed for precise detection and visualization of TCF19 in biological samples. This conjugate combines the specificity of anti-TCF19 antibodies with the fluorescent properties of FITC, enabling applications such as immunofluorescence microscopy, flow cytometry, and immunohistochemistry .

Immunofluorescence (IF)

FITC-conjugated TCF19 antibodies enable direct visualization of TCF19 localization in cultured cells. For example:

• Protocol: Dilute 1:500 in PBS with 10% fetal bovine serum (FBS); incubate 1 hour in the dark .

• Validation: Demonstrated in CHO cells expressing recombinant TCF19-tagged proteins, with low background signal .

Western Blotting (WB) and ELISA

• Detects TCF19 in lysates from lung cancer cells (e.g., H460, Hop62) .

• Validated in INS-1 β-cells and human islets for studying proliferation and DNA damage pathways .

Functional Studies

• Cancer Research: TCF19 promotes tumorigenesis in lung adenocarcinoma by activating the RAF/MEK/ERK pathway .

• Diabetes: TCF19 overexpression in β-cells increases nucleotide incorporation and inflammatory gene expression .

Specificity

• Binds selectively to human TCF19 epitopes (e.g., residues 12–250) .

• Recognizes both endogenous and overexpressed TCF19 in HepG2, H460, and INS-1 cells .

Reproducibility

• Lot Consistency: Protein G-purified batches show >95% purity .

• Knockdown Validation: Reduced signal in shTCF19-treated lung cancer cells (H446, PC-9) .

Cross-Reactivity

• No cross-reactivity with rodent TCF19 isoforms (lacks the PHD finger domain) .

Role in Cancer

Epigenetic Regulation

• TCF19 binds H3K4Me3 via its PHD finger, recruiting co-repressors to modulate gluconeogenic genes .

• Disruption of this interaction reduces hepatic cell proliferation by 40–60% .

Limitations and Considerations

• Photobleaching: FITC fluorescence degrades under prolonged light exposure .

• Species Specificity: Limited to human samples in most commercial variants .

• Dilution Optimization: Requires empirical adjustment for non-standard assays (e.g., FFPE tissues) .

Future Directions

TCF19 FITC-conjugated antibodies are critical for dissecting its dual roles in oncogenesis and metabolic regulation. Ongoing studies focus on:

• Targeting TCF19-H3K4Me3 interactions for cancer therapy .

• Validating TCF19 as a biomarker in diabetes-associated β-cell dysfunction .