TCN1 Antibody
Description
Introduction to TCN1 Antibody
TCN1 antibodies target the Transcobalamin I protein, encoded by the TCN1 gene. This protein binds vitamin B12 (cobalamin) in serum and biological fluids, facilitating its transport and protecting it from degradation in acidic environments . Antibodies against TCN1 are critical for:
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Quantifying TCN1 expression in tissues (e.g., cancer vs. normal).
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Investigating its role in tumorigenesis, metastasis, and chemoresistance.
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Developing diagnostic and prognostic assays.
Applications in Biomedical Research
TCN1 antibodies are utilized across multiple experimental platforms:
Therapeutic Insights
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Chemosensitivity: Low TCN1 expression in hypopharyngeal squamous cell carcinoma (HPSCC) predicts better response to neoadjuvant chemotherapy (NACT) (p < 0.001) .
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Immune Modulation: TCN1 interacts with immune checkpoints (e.g., CD276) and chemokines (CXCL1, CXCL5), suggesting roles in immune cell recruitment .
Role in Cancer Prognosis and Therapy
TCN1 antibodies have identified the protein as a dual-function biomarker:
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Research suggests that higher placental TCN1 protein abundance is associated with elevated cord blood vitamin B12 concentrations, indicating a potential role in vitamin B12 transport to the fetus. PMID: 27577703
- The rs526934 variant in the TCN1 gene has been linked to an increased risk of developing gastric cancer. PMID: 26959381
- Patients with cobalamin deficiency often exhibit decreased levels of holotranscobalamin. PMID: 24057896
- Studies have elucidated the structural basis for universal corrinoid recognition by the cobalamin transport protein haptocorrin. PMID: 23846701
- TCN1 gene expression appears to be implicated in disease progression in patients with middle ear cholesteatoma. PMID: 23670528
- A comparison of human and rainbow trout cobalamin-binding protein has been conducted. PMID: 22872637
- Elevated B(12) concentrations observed in autoimmune lymphoproliferative syndrome patients have been attributed to increased lymphocyte expression of haptocorrin. PMID: 22306884
- Stromal expression of KRT15, TCN1, and HOXB13 has been found to be significantly correlated with tumor grade, stromal hypercellularity, mitotic activity, and microscopic borders. PMID: 21574054
- Two novel mutations, each causing a premature stop codon, have been identified as a genetic basis for TC I deficiency. PMID: 19686235
- A comparative analysis of cobalamin binding kinetics has been performed. PMID: 11788601
- Research indicates no association between the MTHFR A1298C and transcobalamin C776G genetic polymorphisms and hyperhomocysteinemia in thrombotic disease. PMID: 12590948
- Investigations have explored the interactions of COBALAMIN BINDING proteins with a number of ligands. PMID: 17487979
- A study in a Brazilian population suggests that maternal folate-related polymorphisms (CBS, MTR, RFC-1, and TC) do not influence trisomy 21 susceptibility. PMID: 19729796
Q&A
Basic Research Questions
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What is TCN1 and why is it important in biomedical research?
TCN1 (Transcobalamin-1) is a vitamin B12-binding protein that regulates cobalamin homeostasis. It plays multiple roles in maintaining basic functions of cell metabolism and proliferation, particularly in hematopoiesis and neural development . The protein has a reported length of 433 amino acid residues and a mass of approximately 48.2 kDa in humans . It is known to bind vitamin B12 with femtomolar affinity and protects it from the acidic environment of the stomach .
Recent studies have shown elevated TCN1 expression in various cancer types, including lung adenocarcinoma (LUAD), colon cancer, hypopharyngeal squamous cell cancer, breast cancer, and gastric cancers, suggesting its potential role as a biomarker in cancer research . The overexpression of TCN1 in tumor tissues has been linked to tumorigenesis and poor biological behavior, making it a promising target for cancer research .
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What are the common applications for TCN1 antibodies in research?
TCN1 antibodies are utilized in several research applications:
The most widely used application is Western Blot, followed by ELISA and Immunohistochemistry . The appropriate application depends on the specific research question being addressed.
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How should I select the appropriate TCN1 antibody for my research?
When selecting a TCN1 antibody, consider the following factors:
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Species reactivity: Ensure the antibody recognizes TCN1 in your species of interest (common options include Human, Mouse, Rat)
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Clonality: Monoclonal antibodies offer high specificity for a single epitope, while polyclonal antibodies recognize multiple epitopes and may provide stronger signals
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Application validation: Verify the antibody has been validated for your specific application (WB, IHC, ELISA, etc.)
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Host species: Choose a host species that won't cross-react with other antibodies in your experimental design
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Epitope recognition: For specialized experiments, consider antibodies recognizing specific domains of TCN1
Rigorous validation using positive and negative controls is essential to ensure antibody specificity and reliability in your experimental system.
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