tdrd9 Antibody
Description
Definition and Function
The TDRD9 antibody is a polyclonal or monoclonal immunoglobulin designed to specifically bind to the Tudor domain-containing protein 9 (TDRD9), a RNA helicase critical for piRNA (PIWI-interacting RNA) biogenesis and germ cell development. TDRD9 is primarily expressed in the germline, where it silences transposable elements like Line-1 retrotransposons, ensuring genome stability . In cancer, its overexpression in subsets of lung adenocarcinoma and melanoma correlates with hypomethylation of its CpG island, indicating a potential oncogenic role .
Research Applications
a. Cancer Prognosis
TDRD9 antibodies are used to detect its overexpression in 15%–31% of lung adenocarcinoma and melanoma samples, correlating with poor prognosis . Immunohistochemistry (IHC) and Western blotting reveal its role in protecting tumor cells from replication stress via DNA-PK activation .
b. Germline Development
In knockout mice, TDRD9 deficiency leads to Line-1 activation and male sterility, as shown by immunofluorescence assays detecting nuclear defects in germ cells .
c. Human Infertility
A frameshift mutation in TDRD9 causes non-obstructive azoospermia (NOA) in men. Antibody staining confirmed protein presence but revealed disrupted function, mimicking mouse phenotypes .
Technical Considerations
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Sensitivity: Western blot detection requires 1.0 μg/ml antibody concentration (Novus NBP1-57116) .
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Cross-reactivity: Antibodies-Online ABIN1385847 reacts with human, mouse, rat, and dog TDRD9 .
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Storage: Most antibodies require -20°C storage; avoid freeze-thaw cycles .
Key Research Findings
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
