TECPR1 Antibody, HRP conjugated

Overview

The TECPR1 Antibody, HRP conjugated is a polyclonal antibody raised against recombinant human Tectonin beta-propeller repeat-containing protein 1 (TECPR1). Designed for immunodetection, it targets the 199–487 amino acid region of the protein. This antibody is conjugated with horseradish peroxidase (HRP), enabling its use in enzyme-linked immunosorbent assays (ELISA) and other immunoassays requiring enzymatic detection. It is produced in rabbits and exhibits specificity for human TECPR1, with no reported cross-reactivity with other species .

Applications

The antibody is primarily validated for ELISA, though its compatibility with other techniques (e.g., Western blot, immunohistochemistry) may be inferred from TECPR1’s established role in autophagy research .

Research Findings

TECPR1 is a lysosomal protein critical for autophagy and selective degradation of damaged organelles or pathogens. Key findings include:

• Role in Autophagy: TECPR1 recruits LC3C-positive autophagosomes to lysosomes via its LIR motif, promoting protein aggregate clearance .

• Damage Sensing: Binds sphingomyelin exposed on damaged membranes, facilitating LC3/GABARAP lipidation independently of ATG16L1 .

• Pathogen Defense: Required for phagophore formation against bacterial pathogens like Salmonella Typhimurium .

While this specific HRP-conjugated antibody has not been cited in published studies, its design aligns with established TECPR1 detection methods. For example:

• ELISA: Used to quantify TECPR1 in lysosomal fractions .

• Western Blot: Detects a 130 kDa band in human lysates .

References

1. Biocompare. Anti-TECPR1 Antibody Products.

2. Nature. TECPR1 promotes aggrephagy by direct recruitment of LC3C.

3. Cell Signaling Technology. TECPR1 (D6C10) Rabbit mAb #8097.

4. EMBO Press. TECPR1 conjugates LC3 to damaged endomembranes.

5. Dempfle, L. Revealing the role of TECPR1 in mammalian autophagy.

6. PMC. TECPR1 is activated by damage-induced sphingomyelin exposure.

7. Cepham Life Sciences. TECPR1 Antibody, HRP conjugated.

8. PMC. An ATG12-ATG5-TECPR1 E3-like complex regulates lysosome repair.