TFB1M Antibody, FITC conjugated
Description
Key Functional Roles of TFB1M
TFB1M is a mitochondrial methyltransferase that dimethylates 12S rRNA at residues A1583 and A1584, critical for ribosomal small subunit assembly . It also regulates mitochondrial DNA transcription via interactions with POLRMT and TFAM .
Experimental Use Cases
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Mitochondrial Ribosome Assembly Studies: Knockdown of TFB1M disrupts mitochondrial small subunit (28S) assembly, reducing ATP production and impairing mitochondrial protein synthesis .
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Cancer Research: TFB1M overexpression in hepatocellular carcinoma (HCC) promotes aerobic glycolysis (Warburg effect) and metastasis by altering mitochondrial metabolism .
Recommended Dilutions
| Application | Dilution Range | Validated Cell Lines |
|---|---|---|
| Immunofluorescence | 1:400–1:1600 | HepG2 (human liver cancer) |
| Immunoprecipitation | 0.5–4.0 µg/1–3 mg lysate | HepG2 |
Comparative Analysis of TFB1M Antibodies
The table below contrasts the FITC-conjugated TFB1M antibody with other commercially available variants:
Research Findings Using TFB1M Antibodies
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Mitochondrial Translation: Knockdown of TFB1M reduced puromycin incorporation into nascent mitochondrial proteins by 60%, confirming its role in translation .
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Metabolic Reprogramming: TFB1M overexpression in HCC cells increased lactate production by 2.5-fold and decreased oxygen consumption by 40%, linking it to the Warburg effect .
Limitations and Considerations
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Loss of TFB1M leads to mitochondrial dysfunction, resulting in impaired insulin secretion and diabetes. PMID: 24916378
- Deficiency in TFB1M and subsequent impaired mitochondrial function contribute significantly to the pathogenesis of type 2 diabetes. PMID: 21195351
- Endurance training has been shown to influence the mRNA levels of TFB1M and TFB2M. PMID: 19681768
- This transcription factor effectively activates the transcription of human mitochondrial DNA. PMID: 12068295
- Human mitochondrial transcription factor B1 (TFB1M) methylates ribosomal RNA at a conserved stem-loop. PMID: 12496758
- TFB1M interacts with the C-terminal activation region of h-mtTFA and stimulates transcription independently of its RNA methyltransferase activity. PMID: 12897151
- TFB1M is identified as a nuclear-encoded modifier gene for the phenotypic expression of the deafness-associated homoplasmic A1555G mutation in the mitochondrial 12S rRNA gene. PMID: 15110318
- Distinct, yet possibly coordinated functions of mtTFB1 and mtTFB2 in mitochondrial gene expression and biogenesis have been observed. PMID: 17557812
- Research suggests that DNA variants in TFB1M do not contribute to the risk of Parkinson's disease. PMID: 18980857
- A study examined the variation in TFAM, TFB1M, and TFB2M genes in cardiac hypertrophy. PMID: 19096125
- rRNA methyltransferase activity is essential for the induction of mitochondrial biogenesis by TFB1M, but not TFB2M. PMID: 19417006
