TFDP2 Antibody, HRP conjugated

Antibody Characteristics

The HRP-conjugated TFDP2 antibody is a rabbit polyclonal antibody targeting the N-terminal or internal regions of TFDP2. Key features include:

This antibody is purified via antigen affinity or Protein G methods, ensuring high specificity .

Western Blot (WB)

The HRP-conjugated antibody detects TFDP2 in WB with high sensitivity. Recommended dilutions and protocols:

Key Findings:

• TFDP2 upregulation is linked to viral proliferation (e.g., PRRSV) and erythroid differentiation .

• Knockdown of TFDP2 reduces cyclin A expression and arrests cells in S-phase .

ELISA

The HRP-conjugated antibody is validated for ELISA, enabling quantitative TFDP2 detection:

Mechanistic Insights from TFDP2 Research

TFDP2 forms heterodimers with E2F transcription factors to regulate:

1. Cell Cycle Progression:

   • Promotes G1-S phase transition via cyclin A and DHFR genes .

   • Inhibits terminal differentiation in erythroblasts by repressing E2F2 target genes .

2. Pathological Roles:

   • PRRSV Infection: TFDP2 upregulation by viral N protein enhances viral replication .

   • Erythropoiesis: TFDP2 knockdown disrupts hemoglobin synthesis and delays enucleation .

3. Immune Infiltration:

   • Correlates with B cells, neutrophils, and dendritic cells in pancreatic adenocarcinoma (PAAD) .

Western Blot Protocol

1. Sample Preparation:

   • Lysate cells in RIPA buffer, resolve proteins via SDS-PAGE.

2. Membrane Transfer:

   • Transfer to nitrocellulose, block with 5% milk/TBS.

3. Antibody Incubation:

   • Primary: 0.5 μg/mL HRP-TFDP2 antibody (4°C, overnight).

   • Secondary: Goat anti-rabbit IgG-HRP (1:5000, RT, 1.5 h).

4. Detection:

   • Use ECL reagent; visualize at 49 kDa .

ELISA Protocol

1. Coating:

   • Coat plates with TFDP2 antigen (50 ng/well).

2. Blocking:

   • Block with 1% BSA/PBS.

3. Detection:

   • Incubate with HRP-TFDP2 antibody (1:500–1:1000), develop with TMB substrate .

Comparison with Non-HRP Conjugated Antibodies

Clinical and Diagnostic Relevance

TFDP2 antibodies, including HRP-conjugated variants, are critical in:

• Cancer Research: Prognostic biomarker for PAAD and immune cell infiltration .

• Viral Pathogenesis: Monitoring TFDP2 upregulation in PRRSV-infected cells .

• Hematopoiesis: Studying erythroid differentiation defects .

Critical Considerations

1. Optimal Dilution:

   • Titrate antibody for each assay type to minimize background .

2. Cross-reactivity:

   • Verify reactivity with target species (e.g., human vs. mouse) .

3. Storage Stability:

   • Avoid freeze-thaw cycles; store at -20°C .

This antibody offers a robust tool for studying TFDP2’s role in cell cycle regulation, disease pathogenesis, and therapeutic targeting. Its HRP conjugation streamlines detection workflows, enabling high-throughput and quantitative analyses.