THOC6 Antibody, Biotin conjugated
Description
THOC6 as a Biomarker in Glioma
THOC6 has emerged as a critical diagnostic and prognostic biomarker for glioma, a malignant brain tumor. Research indicates that THOC6 expression correlates strongly with glioma progression and poor patient outcomes . Key findings include:
Table 1: Pathways associated with THOC6 expression in glioma
| Pathway | P-value (adjusted) | Description |
|---|---|---|
| IL-17 signaling pathway | 0.034 | Regulates immune responses and inflammation |
| Rho GTPase signaling | 0.011 | Controls cytoskeleton dynamics and migration |
| DNA repair | 0.017 | Maintains genomic stability |
| Extracellular matrix | 0.011 | Modulates tumor microenvironment |
Biotin Conjugation in Immunoreagents
Biotinylated antibodies, including the THOC6-specific variant, are widely used in immunodetection assays due to their compatibility with streptavidin/avidin-based systems. Key advantages include:
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Signal amplification: Multiple biotin molecules on secondary antibodies enhance sensitivity in techniques like Western blotting and immunohistochemistry (IHC) .
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Versatility: Biotinylated antibodies are compatible with enzymatic (e.g., HRP-streptavidin) or fluorescent (e.g., Alexa Fluor-streptavidin) detection systems .
Applications of THOC6 Antibody, Biotin Conjugated
This antibody is primarily employed in glioma-related research and diagnostics. Example applications include:
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Immunohistochemistry (IHC): Detects THOC6 protein in tumor tissue sections to assess prognosis .
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Western blotting: Validates THOC6 expression levels in glioma cell lines (e.g., U87 MG) .
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Flow cytometry: Monitors THOC6 expression in immune-infiltrated glioma cells .
Table 2: Product specifications for biotin-conjugated secondary antibodies
| Catalog Number | Host Species | Target Species | Cross-Reactivity |
|---|---|---|---|
| 20190 | Donkey | Mouse IgG | Multiple species |
| 20191 | Donkey | Rabbit IgG | Human, mouse, etc. |
| 20184 | Goat | Mouse IgG | Human, rabbit |
Research Findings on THOC6 Antibody Utility
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Diagnostic accuracy: ROC curve analysis shows THOC6 has an AUC of 0.915 for glioma diagnosis, indicating high specificity .
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Therapeutic potential: Molecular docking studies suggest THOC6 interacts with glioma drugs like temozolomide (docking energy: −7.1 kcal/mol) .
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Immune infiltration: High THOC6 expression correlates with increased infiltration of neutrophils and dendritic cells in glioblastoma (GBM) .
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Research indicates that three unrelated patients with bi-allelic mutations in THOC6 exhibit intellectual disability and additional clinical features. PMID: 27102954
- In addition to confirming the pathological nature of THOC6 by identifying an independent homozygous, apparently loss-of-function allele in a patient with a compatible phenotype, our data expand the THOC6-related phenotype to include previously unreported imperforate anus and undescended testicles. PMID: 26739162
