mpst-1 Antibody

Western Blotting (WB)

MPST-1 antibodies are widely used to quantify MPST protein levels. For example:

• Clone H-11 detects MPST in 10 µg SHSY-5Y cell lysate at 0.5 µg/mL .

• Clone NBP1-54734 identifies MPST in human, mouse, and rat liver lysates at 1.0 µg/mL .

Immunohistochemistry (IHC)

• Clone 1H10.1 effectively labels MPST in human heart and kidney tissues at 1:50 dilution .

• Clone NBP1-82617 demonstrates granular cytoplasmic staining in human liver sections (1:500–1:1000) .

Immunofluorescence (IF)

• Clone A04609-1 visualizes MPST in PC3 cells using DyLight®488-conjugated secondary antibodies .

• Clone NBP1-82617 localizes MPST to mitochondria in A-431 cells .

Flow Cytometry

• Clone A04609-1 distinguishes MPST-positive SH-SY5Y cells from isotype controls .

Role in Cyanide Detoxification

MPST catalyzes sulfur transfer to cyanide, converting it to thiocyanate. Antibodies like H-11 and 1H10.1 are essential for studying this pathway in toxicology models .

HIF-1α Regulation

• 1H10.1-mediated studies reveal MPST’s interaction with cystathionine β-synthase (CBS) in regulating hypoxia-inducible factor-1α (HIF-1α) stability via persulfidation of PHD2 .

Astrocyte Dysfunction in Aging

• MPST expression is linked to astrocyte dysfunction in aging brains, as shown using 1H10.1 in murine and human models .

Mitochondrial Localization

• NBP1-82617 highlights mitochondrial MPST staining in A-431 cells, supporting its role in redox homeostasis .

Comparative Performance of MPST-1 Antibody Clones