TIE1 Fc Human

TIE1 Fc Human Recombinant
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Cat. No.
BT28685
Source
Insect Cells.
Synonyms
Tyrosine kinase with immunoglobulin-like and EGF-like domains 1, JTK14, TIE, TIE1.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 90.0% as determined by:
(A) Analysis by RP-HPLC.
(B) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Research Applications

TIE1 Fc Human serves as critical tool in:

  • Angiogenesis Studies: Modulates endothelial cell signaling via TIE1-TIE2 receptor complexes

  • Ligand Interaction Analysis: Despite TIE1 being an orphan receptor, it influences angiopoietin/TIE2 signaling pathways

  • Cancer Research: Used to investigate TIE1's role in tumor progression through mechanisms like Basigin-mediated MMP activation

Key Experimental Uses:

ApplicationModel SystemOutcome Measure
Endothelial MigrationHUVEC cells Flow cytometry quantification
Lymphatic DevelopmentZebrafish embryos RNA-seq analysis of FOXO1 pathway
Cancer MetastasisCervical xenografts MMP2/9 expression levels

Oncogenic Role in Cervical Cancer

  • Proliferation: Overexpression increases colony formation by 2.1× in SiHa cells

  • Metastasis: Enhances pulmonary metastasis incidence by 67% in mouse models

  • Mechanism: Stabilizes Basigin to upregulate MMP2/9 (p<0.01 vs controls)

Lymphatic Regulation

  • Gene Regulation: Controls 1,917 genes in zebrafish lymphatic ECs, including sox18 and foxo1

  • Developmental Impact: tie1 knockout causes lethal edema with 100% penetrance in murine models

Signaling Dynamics

  • Receptor Cooperation: Forms heterocomplexes with TIE2 to modulate angiopoietin responses

  • Kinase Activity: Despite minimal intrinsic kinase function, regulates endothelial quiescence via FOXO1

Clinical Implications

Recent studies identify TIE1 as:

  1. Prognostic Marker: High expression correlates with poor survival in cervical cancer (HR=2.34, p=0.008)

  2. Therapeutic Target: Basigin inhibition reverses TIE1-driven metastasis in vivo

  3. Lymphatic Disease Link: Loss-of-function mutations cause primary lymphedema in humans (OR=8.9, p=3×10^-5)

Product Specs

Introduction
TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/Tek are receptor tyrosine kinases (RTKs) that share a unique structure. This structure includes two immunoglobulin-like domains surrounding three epidermal growth factor (EGF)-like domains, followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are primarily found on endothelial and hematopoietic progenitor cells, playing crucial roles in angiogenesis, vasculogenesis, and hematopoiesis. Human TIE-1 cDNA encodes a precursor protein consisting of 1124 amino acid (aa) residues. This precursor protein includes an 18-residue signal peptide, a 727-residue extracellular domain, and a 354-residue cytoplasmic domain. While two ligands, angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2), have been identified for TIE-2, no ligand has been found for TIE-1 thus far.
Description
Recombinant human TIE-1 Fc Chimera, produced in baculovirus, is a homodimeric glycoprotein. It consists of 749 amino acids fused with the Fc region of human IgG1, resulting in a total molecular mass of 250 kDa. The monomeric form of human TIE-1/Fc has a calculated molecular mass of approximately 105 kDa. However, due to glycosylation, it migrates as an approximately 125 kDa protein on SDS-PAGE under reducing conditions. Purification of the TIE1 Fc Chimera is achieved through proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
TIE-1 Fc Chimera was lyophilized from a sterile solution containing 20mM Tris, 0.5M NaCl, and 10% Sucrose at a concentration of 1 mg/ml.
Solubility
To reconstitute the lyophilized TIE-1 Fc Chimera, it is recommended to dissolve it in sterile water at a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions.
Stability
Lyophilized sTIE-1 remains stable at room temperature for up to 3 weeks; however, it is recommended to store it desiccated below -18°C. Once reconstituted, TIE-1 should be stored at 4°C for 2-7 days. For long-term storage, freezing below -18°C is recommended. To enhance stability during long-term storage, adding a carrier protein (0.1% HSA or BSA) is advisable. Avoid repeated freeze-thaw cycles.
Purity
The purity of this product is greater than 90.0%, as determined by: (A) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) analysis. (B) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
Synonyms
Tyrosine kinase with immunoglobulin-like and EGF-like domains 1, JTK14, TIE, TIE1.
Source
Insect Cells.

Product Science Overview

Introduction

TIE1 Fc (Human Recombinant) is a recombinant protein that combines the extracellular domain of the human TIE1 receptor with the Fc region of human IgG1. This fusion protein is used in various research applications, particularly in the study of angiogenesis and vascular biology.

Structure and Composition

The TIE1 receptor, also known as tyrosine kinase with immunoglobulin and EGF homology domains 1, is a receptor tyrosine kinase (RTK) that plays a crucial role in vascular development and integrity. The TIE1 receptor consists of:

  • Extracellular domain: Contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains, and three fibronectin type III-like repeats.
  • Transmembrane domain: Anchors the receptor in the cell membrane.
  • Cytoplasmic domain: Contains a split tyrosine kinase domain responsible for signal transduction .

The Fc region of human IgG1 is fused to the extracellular domain of TIE1 to create the recombinant protein. This fusion enhances the stability and solubility of the protein, making it easier to purify and use in various assays.

Production and Purification

TIE1 Fc (Human Recombinant) is typically produced in mammalian cell lines, such as Chinese hamster ovary (CHO) cells or mouse myeloma cells (NS0). The recombinant protein is secreted into the culture medium, from which it is purified using affinity chromatography techniques. The final product is often lyophilized for long-term storage and can be reconstituted in a suitable buffer before use .

Applications

TIE1 Fc (Human Recombinant) is used in a variety of research applications, including:

  • Angiogenesis studies: Investigating the role of TIE1 in blood vessel formation and maintenance.
  • Vascular biology: Understanding the signaling pathways involved in vascular development and integrity.
  • Drug development: Screening potential therapeutic agents that target the TIE1 receptor or its signaling pathways.

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Cat. No.
BT28737
Source
CHO Cells.
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