tin-44 Antibody
Description
Overview of TIMM44 Antibodies
TIMM44 antibodies target the translocase of inner mitochondrial membrane 44 homolog, a protein critical for mitochondrial function. TIMM44 facilitates protein import into the mitochondrial matrix by acting as a peripheral membrane protein associated with the TIM23 complex . It recruits mitochondrial heat shock protein 70 (HSP70) to drive ATP-dependent protein translocation . Dysregulation of TIMM44 is linked to mitochondrial dysfunction and diseases such as diabetes and familial oncocytic thyroid carcinoma .
3.1. Functional Studies
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Mitochondrial Protein Import: TIMM44 antibodies validate its role in recruiting HSP70 to the TIM23 complex, essential for protein translocation .
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Disease Models: Upregulated TIMM44 expression in diabetic kidney models and thyroid carcinoma highlights its pathological relevance .
3.2. Techniques
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Western Blot (WB): Detects TIMM44 at ~45 kDa in lysates from human, mouse, and rat tissues .
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Immunofluorescence (IF): Localizes TIMM44 to the mitochondrial inner membrane in cells like L929 and U-2 OS .
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Immunoprecipitation (IP): Used to study TIMM44 interactions with HSP70 and TIM23 complex components .
4.2. Clinical Implications
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Diabetes: TIMM44 overexpression in diabetic murine kidneys correlates with mitochondrial dysfunction .
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Cancer: A TIMM44 mutation is associated with familial oncocytic thyroid carcinoma, suggesting a role in tumorigenesis .
Validation and Quality Control
| Parameter | Proteintech 13859-1-AP | Abclonal A17446 | Novus SR4295 |
|---|---|---|---|
| Peer-Reviewed Citations | 21+ (WB), 5+ (IF) | N/A | 1+ (WB) |
| Specificity | Confirmed via KO validation | ELISA/WB confirmed | Recombinant peptide validation |
Storage: All antibodies are stable at -20°C in glycerol-containing buffers .
Product Specs
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
