TIR1 Antibody
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Product Specs
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Made-to-order (14-16 weeks)
Synonyms
TIR1 antibody; FBL1 antibody; WEI1 antibody; At3g62980 antibody; T20O10.80 antibody; Protein TRANSPORT INHIBITOR RESPONSE 1 antibody; Weak ethylene-insensitive protein 1 antibody
Target Names
TIR1
Uniprot No.
Target Background
Function
The TIR1 protein serves as an auxin receptor, mediating the proteasomal degradation of Aux/IAA proteins and subsequently influencing auxin-regulated transcription. The SCF(TIR1) E3 ubiquitin ligase complex plays a crucial role in the auxin-mediated signaling pathway, which regulates various developmental processes, including root and hypocotyl growth, lateral root formation, cell elongation, and gravitropism. Notably, TIR1 appears to enable pericycle cells to overcome G2 arrest prior to lateral root development. Furthermore, it has been implicated in ethylene signaling within roots and contributes to plant sensitivity to the virulent bacterial pathogen *P. syringae*.
Gene References Into Functions
- In vitro and in vivo protein-protein interaction assays have demonstrated that nitric oxide (NO) enhances the binding of ASK1 to CUL1 and TIR1/AFB2, essential components for the assembly of the SCF(TIR1/AFB2) complex. PMID: 30031268
- The miR393-TIR1 molecular regulatory pathway is believed to be a critical component of the regulatory control over shoot regeneration from in vitro culture. PMID: 29241559
- The auxin perception mediated by TIR1/AFB-Aux/IAA complexes is directly involved in the rapid acidification of cell walls and subsequent growth of Arabidopsis hypocotyls. PMID: 27627746
- HSP90, a molecular chaperone, stabilizes the auxin receptor TIR1, ensuring the flexibility and adaptability of auxin responses. PMID: 28532230
- Research suggests that oligomerization of TIR1 is a novel regulatory mechanism involved in the control of auxin-mediated plant patterning and development. PMID: 27618443
- Studies have shown that HSP90 masks point mutations within the auxin receptor TIR1, thereby buffering auxin-responsive phenotypes. PMID: 27816945
- Elevated temperatures promote rapid accumulation of the TIR1 auxin co-receptor, an effect that is dependent on the molecular chaperone HSP90. This finding highlights the integrated roles of HSP90 and SGT1 in temperature and auxin signaling. PMID: 26728313
- Overexpression of a miR393-resistant TIR1 gene (mTIR1) in Arabidopsis has been shown to enhance salt stress tolerance. PMID: 25336111
- Auxin-binding protein 1 (ABP1) acts as a negative regulator of the SCF(TIR1/AFB) pathway. PMID: 24051655
- Variations within the leucine-rich repeat domain of the TIR1 auxin coreceptor can alter the properties of the SCF(TIR1) complex. PMID: 23539280
- Elevated auxin levels, and even suboptimal levels, require the activity of the SKP-Cullin-F-box(TIR1/AFB) (SCF(TIR1/AFB))-dependent pathway for the degradation of PIN proteins. PMID: 23211744
- Mutations in the ASA1 gene and the TIR1/AFBs auxin receptor genes impair the inhibitory effect of 5 μM MeJA on PIN2 endocytosis and enhance the reduction effect of 50 μM MeJA on the plasma membrane accumulation of PIN2. PMID: 21466556
- The TIR1 auxin receptor family is not involved in auxin-induced H(+)-ATPase phosphorylation. PMID: 22492846
- miR393 and secondary siRNAs regulate the expression of the TIR1 gene. PMID: 21828251
- The interaction between miR393 and its target indicates a fine-tuning mechanism for the miR393-TIR1 module, essential for auxin responses in plant development. PMID: 22042293
- TIR1 and AFB2 proteins exhibit distinct biochemical activities, with TIR1 and AFB2 being the dominant auxin receptors in the seedling root. PMID: 20018756
- Biochemical studies reveal that IAA1/AXR5 interacts with TIR1 in an auxin-dependent manner. PMID: 15546359
- Research indicates that TIR1 acts as an auxin receptor, mediating the degradation of Aux/IAA proteins and subsequently influencing auxin-regulated transcription. PMID: 15917797
- TIR1 is identified as an auxin receptor mediating transcriptional responses to auxin. PMID: 15917798
- Crystal structures of the Arabidopsis TIR1-ASK1 complex have been determined, both in its free state and in complexes with three different auxin compounds and an Aux/IAA substrate peptide. PMID: 17410169
- Modulation of auxin sensitivity by phosphate (Pi) availability has been found to depend on the auxin receptor TRANSPORT INHIBITOR RESPONSE1 (TIR1) and the transcription factor AUXIN RESPONSE FACTOR19 (ARF19). PMID: 19106375
- The accumulation of SCFTIR1 in Arabidopsis is regulated by the COP9 signalosome and the 26S proteasome. PMID: 19147500
- Auxin enhances the TIR1-mediated ubiquitination of SHY2 and IAA12. PMID: 19309453
Database Links
Subcellular Location
Nucleus.
Tissue Specificity
Expressed in roots, stems, leaves and flowers. In adult plants, mostly expressed in floral stigma, anther filaments, abscission zones and vascular tissues.
Q&A
Here’s a structured FAQ for TIR1 antibody research, organized by scientific depth and methodology, based on current research findings:
How do I validate TIR1 antibody specificity in plant protein extracts?
Methodological approach:
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Perform immunoblotting using Arabidopsis tir1-1 mutant lines as negative controls .
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Use epitope mapping with synthetic peptides matching TIR1’s auxin-binding pocket (residues 170–473) to confirm antibody recognition .
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Validate via co-immunoprecipitation (Co-IP) with known TIR1 interactors (e.g., ASK1 or Aux/IAA proteins) .
What are key applications of TIR1 antibodies in auxin signaling studies?
Experimental workflows:
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Quantitative Western blotting: Measure TIR1 protein levels in response to auxin treatment (10 µM IAA, 0–60 min) .
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Immunolocalization: Optimize fixation protocols (4% formaldehyde, 20 min) to preserve TIR1 subcellular localization in root tips .
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Pull-down assays: Identify TIR1-interacting partners using crosslinkers like DSP (dithiobis[succinimidyl propionate]) .
How to resolve contradictions in TIR1 oligomerization data across studies?
Analytical framework:
What experimental designs address TIR1 antibody cross-reactivity with AFB homologs?
Optimization strategies:
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Generate chimeric AFB/TIR1 proteins to map antibody binding regions (e.g., LRR domain swaps) .
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Use CRISPR-Cas9 knockout lines (e.g., afb1-5, afb2-3) to isolate TIR1-specific signals .
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Employ competitive ELISA with recombinant AFB proteins to quantify cross-reactivity thresholds .
How to quantify TIR1-mediated Aux/IAA degradation kinetics?
Protocol:
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Treat transgenic lines (e.g., IAA7-luciferase) with 5 µM auxin.
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Collect samples at 0, 2, 5, 10, 20 min post-treatment.
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Use TIR1 antibody (1:1,000 dilution) for Western blotting alongside anti-luciferase antibodies .
Critical controls: Include tir1-1 mutants and auxin transport inhibitors (e.g., NPA).
What mutations in TIR1 affect antibody binding affinity?
Key mutations impacting antibody performance:
Why do TIR1 antibodies show inconsistent results in auxin-treated vs. untreated samples?
Diagnostic checklist:
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