TLE4 Antibody
Description
Introduction to TLE4 Antibody
The TLE4 Antibody is a specialized immunological reagent designed to detect and analyze Transducin-Like Enhancer of Split 4 (TLE4), a transcriptional corepressor protein encoded by the TLE4 gene (HGNC: 11840, NCBI Gene: 7091). This antibody plays a critical role in studying TLE4's involvement in gene regulation, epigenetic modifications, and disease pathways .
TLE4 lacks DNA-binding domains and functions by interacting with transcription factors like Blimp1 and β-catenin/TCF complexes to mediate transcriptional repression. It is implicated in Wnt/β-catenin signaling, immune regulation, and cancer progression .
Key Applications of TLE4 Antibody
Epigenetic and Transcriptional Regulation Studies
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TLE4 Antibody is used to investigate its role in silencing interferon-gamma (IFNG) expression in anergic T cells. Calcium signaling triggers TLE4 recruitment to the IFNG locus, establishing repressive epigenetic marks (e.g., histone modifications) .
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Validates interactions with Blimp1, which recruits TLE4 to regulatory elements in autoimmune and inflammatory contexts .
Cancer Research
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Identifies TLE4 downregulation in papillary thyroid carcinoma (PTC), correlating with tumor progression and immune cell infiltration .
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Demonstrates dual roles: acts as a tumor suppressor in PTC (via JAK-STAT pathway modulation) but promotes colorectal cancer through Wnt/β-catenin signaling .
Neurological and Developmental Studies
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Essential for SIX3-mediated transcriptional repression during retina and lens development .
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Linked to autism spectrum disorder and leukemia in genetic association studies .
Table 1: Key Studies Involving TLE4 Antibody
Clinical and Therapeutic Implications
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Diagnostic Potential: TLE4 expression levels serve as a biomarker for PTC prognosis and immune microenvironment status .
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Therapeutic Targets: Modulating TLE4-Blimp1 interactions could restore immune function in autoimmune diseases .
Challenges and Future Directions
Product Specs
Target Background
- TLE4 loss has been linked to proliferative advantage in leukemic cells, accompanied by an upregulation of a pro-inflammatory signature mediated through aberrant increases in Wnt signaling activity. PMID: 27486062
- Research suggests that TLE4, a potential prognostic biomarker for colorectal cancer, plays a significant role in the development and progression of human colorectal cancer. PMID: 26701208
- PPM1B interacts with Groucho 4 and localizes to DNA in a Groucho-dependent manner. Phosphatase activity is crucial for transcriptional silencing. PMID: 25631048
- Groucho-related gene Grg4 robustly activates the expression of a BMP reporter gene, enhancing and sustaining the upregulation of the endogenous Id1 gene induced by BMP7. PMID: 24099773
- Grg4 displaces coactivators CREB-binding protein/p300 while promoting the recruitment of a corepressor, Grg4. PMID: 22927467
- Grg4 recruits the arginine methyltransferase PRMT5 to chromatin, leading to symmetric H4R3 dimethylation. PMID: 22169276
- Germ cell-specific RBMY and hnRNP G-T proteins are more efficient in stimulating TLE4-T incorporation than somatically expressed hnRNP G protein. PMID: 19893608
- Gbx2 and Otx2 interact with the WD40 domain of Groucho/Tle corepressors. PMID: 17060451
