TLR5 Antibody, HRP conjugated
Description
Definition and Structure
TLR5 antibodies are immunoglobulins targeting the extracellular or intracellular domains of TLR5, a transmembrane protein critical for recognizing bacterial flagellin. HRP conjugation allows enzymatic detection in assays like Western blot (WB), immunohistochemistry (IHC), and flow cytometry (FC).
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Molecular Characteristics:
Key Applications
HRP-conjugated TLR5 antibodies are optimized for diverse experimental workflows:
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Functional Insights:
Antibody Properties
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Clone: Monoclonal (e.g., 85B152.5 for Novus Biologicals NBP1-97728H) .
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Immunogen: Synthetic peptides or recombinant TLR5 extracellular domains (e.g., Ile21-Phe115) .
Performance Data
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Western Blot: Bands at ~97–100 kDa in human A549, THP-1, and RAW 264.7 cells .
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IHC: Localized staining in human intestinal mucosa and liver parenchyma .
Research Findings Using TLR5 Antibodies
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Infection Susceptibility: The TLR5 392STOP polymorphism abolishes flagellin signaling, increasing pneumonia risk (Legionella pneumophila) .
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Liver Regeneration: TLR5 activation enhances hepatocyte proliferation post-hepatectomy via NF-κB/STAT3 pathways .
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Neurodegeneration: Soluble TLR5 ectodomain reduces amyloid-β plaques in Alzheimer’s models by direct binding .
Validation and Quality Control
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Specificity: Verified via knockout controls (e.g., Tlr5−/− mice) .
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Batch Consistency: Protein G purification ensures >90% purity .
Comparative Advantages
Product Specs
Target Background
Toll-like receptor 5 (TLR5) is a pattern recognition receptor (PRR) expressed on the cell surface. It plays a crucial role in activating innate immunity and the inflammatory response. TLR5 recognizes pathogen-associated molecular patterns (PAMPs) and microbe-associated molecular patterns (MAMPs) expressed by pathogens and commensal microbiota, respectively. Ligand binding, such as to bacterial flagellins, triggers recruitment of intracellular adapter proteins MYD88 and TRIF, leading to NF-κB activation, cytokine secretion, and the induction of inflammation. TLR5 is thus essential for maintaining the delicate balance between the intestinal epithelium and enteric microbes, significantly contributing to gut microbiota composition throughout life.
Numerous studies highlight the involvement of TLR5 in various biological processes and disease states. Key findings include:
- Associations between TLR5 SNPs and increased activity in Crohn's disease (CD) patients, correlated with elevated IL-12 and IL-18 levels in ulcerative colitis (UC) patients. PMID: 28139755
- Hsp90 inhibitors suppress TLR5 surface expression and NF-κB activation in response to TLR5 ligands in THP-1 cells, suggesting potential implications for myeloid leukemia treatment. PMID: 29651431
- TLR5 adaptor molecules are critical for the appropriate production of cytokines, chemokines, and pro-labor mediators following TLR ligation. PMID: 28844021
- Newly identified long TLR5 transcripts may negatively regulate TLR5 expression and function. PMID: 28948171
- TLR5 polymorphisms (rs5744168, rs2072493, rs5744174) show lower frequency in Indian Tamils, suggesting evolutionary conservation possibly due to selective pressure from endemic infectious diseases. These polymorphisms were not significantly associated with chronic *H. pylori* infection or its associated phenotypes. PMID: 28742407
- Significant interactions were identified between TLR5 SNPs (rs1640827, rs17163737) and *Helicobacter pylori* infection, offering insights into gastric carcinogenesis. PMID: 28404962
- Differential expression of TLR5, TLR7, and TLR9 was observed between HPV-positive and -negative oropharyngeal squamous cell carcinoma patients, with TLR5 and TLR7 expression correlating with tumor recurrence in HPV-positive cases. PMID: 28856441
- HMGB1-modulated TLR5 signaling contributes to pain hypersensitivity. PMID: 27760316
- TLR5 and TLR9 are susceptibility genes for lupus nephritis (LN), with dysregulated expression in LN patient kidneys. PMID: 28763101
- In a Chinese population, TLR5 genetic variation may not determine susceptibility to HBV-related diseases but may influence the development of severe liver disease. PMID: 28543911
- Distinctive flagellin recognition by human and mouse TLR5. PMID: 27391968
- The TLR5 c.1174C>T variant is associated with protection against death from melioidosis, characterized by lower bacteremia and reduced IL-10 and TNF-α production. PMID: 28475641
- Hormonal modulation of TLR5 expression and interleukin-6 production. PMID: 27651177
- Functional expression of TLR5 in salivary glands and response to flagellin. PMID: 27665969
- Involvement of TLR5 in the pathogenesis and dissemination of esophageal adenocarcinoma via currently unknown mechanisms. PMID: 27392931
- TLR1/2 or TLR5 ligands may sustain the growth and malignant phenotype of mantle cell lymphoma (MCL) cells. PMID: 27123851
- No significant difference in TLR5 genotype distribution between bronchopulmonary dysplasia patients and controls. PMID: 27457283
- Expression of TLR5 and TLR7 in salivary adenoid cystic carcinoma, both on cell membranes and in the cytoplasm. PMID: 26888781
- TLR5 rs5744174 polymorphism may not affect stroke risk, gene expression, or inflammatory cytokines but may influence HDL-C serum levels in ischemic stroke patients. PMID: 27262705
- No alteration of TLR5 mRNA in peripheral mononuclear cells from ankylosing spondylitis or rheumatoid arthritis patients. PMID: 26996117
- In response to *Pseudomonas aeruginosa* or flagellin, EGFR associates with and phosphorylates MUC1-CT, increasing its association with TLR5. PMID: 26645913
- Caveolin-1 is a critical regulator of TLR5 expression. PMID: 26615831
- Interaction of MyD88 TIR domain with TLR5 TIR domain, influencing the solubility of both. PMID: 26548862
- Greater abundance of TLR5 in oral squamous cell carcinoma (OSCC) compared to cutaneous squamous cell carcinoma (CSCC) samples. PMID: 25047824
- No association of genetic polymorphism with duodenal ulcers. PMID: 25995217
- p53-dependent amplification of transcriptional response to TLR5 activation, requiring NF-κB p65 and mediated by multiple mechanisms. PMID: 26220208
- Association of TLR5 variants R392X and N592S with ulcerative colitis (UC). PMID: 25789623
- TLR5 specifically interacts with flagellin and serves as an analytical recognition element for low flagellin concentrations. PMID: 24881544
- Review of TLR5 interactions with adaptive immunity and the microbiota, and the impact of TLR5 gene variation on inflammatory phenotypes. PMID: 25284610
- TLR5 as a link between gut microbiota, adipose tissue inflammation, and obesity. PMID: 25611816
- Flagellin-activated TLR5 in non-small cell lung cancer cells initiates downstream signaling pathways. PMID: 25603867
- TLR5 mediates cytokine production in response to *Toxoplasma gondii*-derived profilin. PMID: 24861338
- Association of TLR5 gene polymorphisms with ulcerative colitis and cytokine homeostasis in North India. PMID: 25789623
- TLR5 recognition of commensal microbiota regulates systemic tumor-promoting inflammation and extramucosal malignant progression. PMID: 25533336
- Heterozygosity for NOD2 and TLR5 risk variants and homozygosity for PTPN2 risk alleles in a Crohn's disease family. PMID: 24901824
- Potential association of TLR5 genetic polymorphisms with decreased susceptibility to Graves' disease in Chinese Cantonese females. PMID: 24830583
- Decreased TLR5 and TLR7 expression in severe asthma. PMID: 24447081
- TLR5 activation by flagellin may induce chemoresistance in multiple myeloma patients with recurrent bacterial infections. PMID: 24709011
- Sle1 transgene disrupts peripheral tolerance checkpoints on antinuclear antigen B cells. PMID: 19828626
- Association of TLR5(rs5744105) variant with altered immune responses (elevated WBC counts) in very low birth weight infants during bacterial infections. PMID: 23867959
- Association of frequent SNPs in TLR5, MyD88, and TIRAP with altered survival in colorectal cancer patients. PMID: 24154872
- Association of low TLR5 expression with non-small cell lung cancer. PMID: 24549739
- Involvement of TLR5 in host defense against *Aspergillus fumigatus*, with the TLR5-Stop SNP as a risk factor for invasive aspergillosis. PMID: 23862689
- Abnormal TLR5 expression in esophageal adenocarcinoma pathogenesis and the role of altered microbiome in Barrett's esophagus complications. PMID: 24221343
- Transient TLR5-induced increase in T cell sensitivity to TCR activation following flagellin exposure. PMID: 24128895
- TLR5 signaling enhances proliferation of regulatory T cells. PMID: 23844139
- Polyethylenimine as a novel TLR5 agonist with potential applications in radiation protection. PMID: 23104900
- Potential association of TLR5 variant L616F with Crohn's disease and influence on responses to bacterial flagellin. PMID: 23593463
- Role of TLR5 in rheumatoid arthritis angiogenesis. PMID: 23666857
- Biological activity of a 2:2 stoichiometry TLR5:flagellin complex. PMID: 23624387
