tmem177 Antibody

Introduction to TMEM177 Antibody

The TMEM177 antibody is a research tool designed to detect the Transmembrane Protein 177 (TMEM177), a mitochondrial inner membrane protein implicated in cellular energy production and cancer progression. This antibody is critical for studying TMEM177’s role in cytochrome c oxidase assembly, immune modulation, and oncogenesis. Below is a comprehensive analysis of its properties, applications, and research findings.

2.1. Types of TMEM177 Antibodies

Multiple formats of TMEM177 antibodies are available:

• Polyclonal Antibodies: Derived from rabbit (e.g., Assay Genie PACO30518 , Sigma HPA053816 ) or mouse (NovoPro 176657 ).

• Monoclonal Antibodies: Mouse IgG1 (NovoPro 176657 ) for higher specificity.

2.2. Validation and Applications

3.1. Mitochondrial Role

TMEM177 is essential for the maturation of mitochondrial cytochrome c oxidase subunit II (COX2), a core component of the electron transport chain . It interacts with COX20 and SCO2 to stabilize newly synthesized COX2 during copper chaperoning, ensuring proper assembly of the CuA site .

3.2. Oncogenic Potential

TMEM177 is upregulated in invasive breast cancer and correlates with advanced tumor stages (TNM), lymph node metastasis, and poor prognosis . Its overexpression drives cancer progression via the AKT pathway, promoting proliferation, invasion, and epithelial-to-mesenchymal transition (EMT) .

4.1. Cancer Biology

The antibody has been used to:

• Detect TMEM177 overexpression in breast cancer tissues (IHC) .

• Study EMT markers (e.g., Snail, E-cadherin) in TMEM177-overexpressing cells .

• Validate TMEM177 knockdown effects on cell migration and proliferation .

4.2. Mitochondrial Studies

• Investigated TMEM177’s role in COX2 biogenesis using Western blotting .

• Co-localized TMEM177 with COX20 in mitochondrial fractions via immunofluorescence .