TMPRSS11A Antibody

Introduction

TMPRSS11A (transmembrane serine protease 11A) is a type II transmembrane serine protease expressed in airway epithelial cells, known for its role in cleaving viral spike proteins, including those of SARS-CoV-2 and influenza A virus (FLUAV) . TMPRSS11A antibodies are critical tools in immunodetection and research, enabling the study of its expression, localization, and function in pathological processes.

Applications

TMPRSS11A antibodies are employed in:

• Western Blotting (WB): To detect protein expression in lysates of transfected cells (e.g., HEK293, A549) or tissue samples.

• Immunohistochemistry (IHC): To localize TMPRSS11A in tracheal or lung epithelial tissues, as demonstrated in FLUAV-infected mice .

• ELISA: For quantitative analysis of TMPRSS11A levels in biological fluids.

Research Findings

• TMPRSS11A Autoactivation: Studies using Western blotting and site-directed mutagenesis revealed that TMPRSS11A undergoes intracellular autocatalytic cleavage at Arg186–Ile187, enabling its activation before reaching the cell surface .

• Viral Pathogenesis: TMPRSS11A antibodies have been used to confirm its role in activating FLUAV hemagglutinin and SARS-CoV-2 spike proteins, underscoring its potential as a therapeutic target .

• Inhibitor Studies: Co-expression of HAI-2 (a serine protease inhibitor) reduces TMPRSS11A activation cleavage, suggesting antibody-based assays could monitor protease-inhibitor interactions .

References

1. Zhang et al. (2020). Intracellular autoactivation of TMPRSS11A, an airway epithelial protease activating viral spike proteins.

2. Shirogane et al. (2018). TMPRSS11A activates the influenza A virus hemagglutinin and the Middle East respiratory syndrome coronavirus spike protein.

3. Biocompare. Anti-TMPRSS11 Antibody Products.

4. Antibodies-Online. TMPRSS11A Antibodies.