TOM2B Antibody

Here’s a structured FAQ for researchers working with TOM2B antibodies, synthesized from academic literature and tailored to experimental design and data interpretation:

Advanced Research Questions

How to resolve contradictory data on TOM2B’s role in viral vs. mitochondrial processes?

• Context-dependent analysis:

  System	TOM2B Function	Key Evidence
  Plant cells	Facilitates viral replication	Interaction with TOM1 helicase promotes viral RNA synthesis
  Mammalian cells	Mitochondrial membrane organization	Co-localization with TOMM20 in immunofluorescence

What computational tools predict TOM2B-protein interaction interfaces?

• Use AlphaFold-Multimer for structural predictions and Rosetta for binding energy calculations, as demonstrated in nanobody design workflows .

• Validate interfaces via alanine-scanning mutagenesis coupled with IP .

How to design epitope-specific antibodies against TOM2B?

• Epitope mapping: Combine phage display libraries with deep mutational scanning to identify immunodominant regions .

• Deimmunization: Use ESM-2 language models to reduce T-cell epitopes while preserving binding affinity .

Key Experimental Parameters for TOM2B Studies

Addressing Data Conflicts

• Orthogonal validation: When Western blot bands conflict with predicted sizes (e.g., 70 kDa vs. 16 kDa), perform size-exclusion chromatography to isolate native complexes .

• Functional rescue: Re-express TOM2B in knockout models to confirm phenotype reversibility .