top-3 Antibody

Anti-Rabbit IgG, HRP-Linked Antibody (#7074)

Supplier: Cell Signaling Technology (CST)
Application: Secondary antibody for western blotting, ELISA, and immunohistochemistry.
Key Features:

• Targets rabbit IgG heavy and light chains with minimal cross-reactivity

• Conjugated to horseradish peroxidase (HRP) for chemiluminescent detection
  Research Impact:

• Used in >45,000 publications (2019–2023)

• Critical for detecting rabbit-derived primary antibodies in protein analysis workflows

Anti-Mouse IgG, HRP-Linked Antibody (#7076)

Supplier: Cell Signaling Technology (CST)
Application: Secondary detection reagent for mouse monoclonal antibodies.
Key Features:

• Binds mouse IgG Fc region with high specificity

• Compatible with multiplex assays due to low cross-species reactivity
  Research Impact:

• Cited in >38,000 studies (2019–2023)

• Essential for oncology and immunology research involving mouse models

Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (#4060)

Supplier: Cell Signaling Technology (CST)
Application: Detects phosphorylated Akt at serine 473, a key node in PI3K/AKT/mTOR signaling.
Key Features:

• Validated in IP, flow cytometry, and immunofluorescence

• Distinguishes phosphorylated Akt from non-phosphorylated forms
  Research Impact:

• Featured in >22,000 cancer metabolism and diabetes studies

• Standard tool for assessing PI3K pathway activation in translational research

Comparative Analysis of Top Antibodies

Industry Context and Trends

The dominance of CST antibodies in research (50% of CiteAb’s top 40 antibodies ) reflects their standardized validation protocols. Meanwhile, therapeutic antibodies like Adalimumab (Humira) and Ofatumumab remain clinical leaders, with 15+ approved indications globally . Emerging antibody-drug conjugates (ADCs) such as Patritumab Deruxtecan (HER3-DXd) highlight next-generation trends, though manufacturing challenges delayed its 2024 FDA approval .

Data Sources and Methodologies

1. CiteAb’s Citation Metrics: Aggregates antibody usage data from >4 million publications .

2. YAbS Database: Tracks 2,900+ therapeutic antibodies, including development status and clinical timelines .

3. Regulatory Databases: FDA/EMA approval records for therapeutic antibodies .