TP53INP2 Antibody, HRP conjugated
Description
Overview of TP53INP2 Antibody, HRP Conjugated
The HRP-conjugated TP53INP2 antibody is a polyclonal rabbit IgG antibody designed to target specific epitopes within the human TP53INP2 protein. It is widely used to study TP53INP2’s roles in autophagy, cancer progression, and transcriptional regulation. Key features include:
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Conjugate: Horseradish peroxidase (HRP) for enzymatic signal amplification.
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Immunogen: Recombinant human TP53INP2 protein (residues 100–220; UniProt ID: Q8IXH6) .
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Host Species: Rabbit.
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Reactivities: Human (primary), with cross-reactivity reported in mouse and rat in some formulations .
Role in Autophagy and Cancer
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Autophagosome Formation: TP53INP2 interacts with LC3-related proteins (e.g., GABARAP) and VMP1 to scaffold autophagosome membranes, a mechanism confirmed via coimmunoprecipitation studies .
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Cancer Progression:
Technical Validation
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Immunohistochemistry: Used to identify elevated TP53INP2 levels in 62.5% of bladder cancer tissues compared to 25% in adjacent non-tumor tissues (p < 0.05) .
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ELISA: Validated for quantitative analysis with recommended dilutions of 0.2–1 µg/mL in western blotting .
Table 2: Primary Applications and Protocols
Critical Considerations:
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Nuclear-to-cytoplasmic translocation of TP53INP2 under autophagy induction requires careful subcellular localization analysis .
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Cross-reactivity with mouse/rat tissues necessitates validation for non-human studies .
Limitations and Future Directions
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- TP53INP2 promotes ribosome biogenesis by facilitating rRNA synthesis at the nucleolus. PMID: 27172002
- A replication study using an independent dataset revealed a significant association between TP53INP2 and ER-negative breast cancer (p = 5.07x10-3). Expression of HP (16q22.2) showed a suggestive association with ER-negative breast cancer in the initial discovery phase (Z = 4.30, p = 1.70x10-5) although this association was not significant after Bonferroni adjustment. PMID: 28957356
- Research indicates a correlation between TP53INP2-related basal autophagy and cell growth and malignant progression in human liposarcoma. PMID: 28131096
- Nucleolus integrity is not essential for both DOR nucleo-cytoplasmic shuttling and DOR function in basal autophagy. PMID: 22750142
- Mutation of conserved hydrophobic residues in region 1 of TP53inp2 reduces transcriptional activity, blocks nuclear exit, and inhibits autophagic activity under autophagy-activated conditions. PMID: 22470510
- DOR plays a crucial role in thyroid hormone-mediated osteoblast differentiation, and a DOR SNP is associated with plasma osteocalcin levels in men. PMID: 21467300
- TP53INP2 is a novel gene implicated in the autophagy of mammalian cells. PMID: 19056683
- Alternative splicing of an exon in the 5' untranslated region of the TP53INP2 gene is a key event downstream of hnRNP A2 that is necessary for cells to invade the extracellular matrix. PMID: 19934309
