TPK2 Antibody
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Product Specs
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Made-to-order (14-16 weeks)
Synonyms
TPK2 antibody; At2g44750 antibody; F16B22.24 antibody; Thiamine pyrophosphokinase 2 antibody; AtTPK2 antibody; EC 2.7.6.2 antibody; Thiamine kinase 2 antibody
Target Names
TPK2
Uniprot No.
Target Background
Function
TPK2 Antibody catalyzes the phosphorylation of thiamine to thiamine pyrophosphate (TPP). TPP serves as an essential cofactor for enzymes involved in glycolysis and energy production. Notably, plant leaves require high levels of TPP for efficient photosynthesis and carbohydrate metabolism.
Gene References Into Functions
- Thiamin pyrophosphokinase activity represents the sole mechanism by which exogenous and de novo synthesized thiamin is converted into the enzyme cofactor Thiamin pyrophosphate. PMID: 17611796
Database Links
Protein Families
Thiamine pyrophosphokinase family
Subcellular Location
Cytoplasm, cytosol.
Tissue Specificity
Expressed in leaves and at lower levels in flowers.
Q&A
FAQs for Researchers on TPK2 Antibody in Academic Contexts
The following FAQs address experimental design, methodological considerations, and advanced research applications of TPK2 antibodies, focusing on yeast (Saccharomyces cerevisiae) and plant (Arabidopsis thaliana) models. Data are synthesized from peer-reviewed studies and technical specifications.
Advanced Research Questions
How to investigate TPK2’s role in glucose-responsive phosphorylation of Jhd2?
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Experimental workflow:
Table 1: TPK2-dependent phosphorylation effects on Jhd2 activity
| Condition | H3K4me3 Level | Jhd2 Phosphorylation | TPK2 Localization |
|---|---|---|---|
| 0.05% glucose | Low | Undetectable | Nuclear |
| 2% glucose | High | Elevated | Cytoplasmic |
| tpk2Δ mutant | Low | Absent | Nuclear |
How to resolve contradictions in TPK2 localization studies?
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Conflict: Tpk2 is reported in both cytoplasm and nucleus.
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Resolution:
What methods confirm TPK2-Jhd2 interaction in vivo?
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Co-IP/MS: Immunoprecipitate Tpk2 and perform LC-MS/MS to identify Jhd2 peptides .
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Genetic epistasis: Compare H3K4me3 levels in tpk2Δ, jhd2Δ, and tpk2Δ jhd2Δ mutants .
Methodological Challenges & Solutions
How to address low signal in TPK2 ChIP-seq?
-
Optimization steps:
Why do TPK2 antibody results vary between yeast and plant studies?
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