TPS03 Antibody

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Product Specs

Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Made-to-order (14-16 weeks)
Synonyms
TPS03 antibody; At4g16740 antibody; dl4395w antibody; FCAALL.18 antibody; Tricyclene synthase antibody; chloroplastic antibody; EC 4.2.3.105; antibody; E)-beta-ocimene synthase antibody; EC 4.2.3.106; antibody; E,E)-alpha-farnesene synthase antibody; EC 4.2.3.46 antibody; Terpenoid synthase 3 antibody; AtTPS03 antibody
Target Names
TPS03
Uniprot No.

Target Background

Function
TPS03 is primarily involved in the biosynthesis of sesquiterpenes (C15). Utilizing farnesyl pyrophosphate (FPP) as a substrate, the primary product is (E,E)-alpha-farnesene, with minor amounts of (Z,E)-alpha-farnesene and (E,E)-beta-farnesene. When using geranyl pyrophosphate (GPP) as a substrate, TPS03 can also synthesize monoterpenes (C10) in vitro, with (E)-beta-ocimene as the major product and (Z)-beta-ocimene and myrcene as minor products.
Gene References Into Functions
  1. Variations in the relative emissions of (E)-beta-ocimene and (E,E)-alpha-farnesene from different accessions have been attributed to allelic variation within two closely related, tandem-duplicated terpene synthase genes, TPS02 and TPS03. [TPS03] PMID: 20463089
Database Links

KEGG: ath:AT4G16740

STRING: 3702.AT4G16740.1

UniGene: At.44857

Protein Families
Terpene synthase family, Tpsb subfamily
Subcellular Location
Cytoplasm. Plastid, chloroplast stroma.
Tissue Specificity
Predominantly expressed in flowers but also in leaves, roots, stems and siliques.

Q&A

Here’s a structured collection of FAQs for researchers working with the TPS03 Antibody, grounded in academic research scenarios and informed by methodological rigor:

Advanced Research Questions

How should data contradictions involving TPS03 Antibody be resolved?

IssueMethodological Approach
Inconsistent stainingCompare antibody performance across multiple fixation methods or antigen retrieval protocols .
Cross-reactivityPerform mass spectrometry or siRNA knockdown to confirm target specificity .

What are best practices for cross-species TPS03 Antibody applications?

  • Validate reactivity using phylogenetic alignment of the target epitope .

  • Clearly state species-specific validation data in methods (e.g., murine vs. human samples) .

How can epitope mapping improve TPS03 Antibody experimental design?

MethodUtility
Peptide array screeningIdentifies exact binding regions to avoid off-target interactions .
Structural modelingPredicts epitope accessibility in folded protein conformations .

What protocols ensure TPS03 Antibody stability in long-term studies?

  • Aliquot antibodies to minimize freeze-thaw cycles .

  • Document storage conditions (e.g., buffer composition, temperature) .

How to validate TPS03 Antibody in multiplex assays?

  • Use spectral deconvolution or sequential staining to avoid cross-talk .

  • Include single-antibody controls to confirm signal specificity .

Key Methodological Considerations

How should researchers address conflicting TPS03 Antibody literature findings?

  • Reanalyze experimental conditions (e.g., antibody dilution, incubation time) .

  • Compare results across independent validation platforms (e.g., CRISPR-Cas9 knockout vs. RNAi) .

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