TPS07 Antibody

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Description

Product Overview

The TPS07 Antibody (Catalog: CSB-PA526508XA01DOA) is a mouse-derived monoclonal antibody. Key details include:

ParameterSpecification
Target AntigenTPS (UniProt ID: O65434)
Host SpeciesMouse
ClonalityMonoclonal
Immunogen SequenceDerived from Arabidopsis thaliana (Mouse-ear cress)
ReactivityArabidopsis thaliana
ApplicationsWestern Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Working DilutionsWB: 0.5–2 µg/mL (1:500–2000); IHC/ICC: 5–20 µg/mL (1:50–200)
Storage ConditionsShort-term: 2–8°C; Long-term: -20°C
FormulationLiquid in PBS (pH 7.4) with 0.05% Proclin-300 and 50% glycerol
SupplierCloud Clone (via Cusabio)

Antibody Architecture

As a monoclonal antibody, TPS07 adopts the canonical Y-shaped structure common to immunoglobulins :

  • Variable Regions (Fab): Bind specifically to the TPS epitope in Arabidopsis.

  • Constant Regions (Fc): Mediate immune effector functions (e.g., binding to protein A/G).

Target Antigen: TPS in Arabidopsis

While the precise role of the TPS07 target protein is not detailed in the provided sources, TPS enzymes in plants are broadly associated with terpene biosynthesis, which influences growth, defense mechanisms, and secondary metabolite production.

Research Applications

The antibody is validated for use in:

  • Western Blot: Detect TPS protein expression in Arabidopsis lysates.

  • Immunohistochemistry: Localize TPS in plant tissue sections.

  • Immunocytochemistry: Study subcellular distribution in cultured cells.

Performance Notes

  • Optimal dilutions must be empirically determined for each experimental setup.

  • Cross-reactivity with non-Arabidopsis species has not been reported.

Comparative Analysis

TPS07 is one of several TPS-targeting antibodies in Cusabio’s catalog, including TPS01, TPS06, and TPS10. Unlike antibodies against human TPS (e.g., tissue polypeptide-specific antigen) , TPS07 is specialized for plant research.

Limitations and Future Directions

  • Current Data Gap: No peer-reviewed studies utilizing TPS07 Antibody were identified in the provided sources. Further validation in published research is needed.

  • Potential Uses: Could be applied to investigate terpene synthase pathways or stress responses in Arabidopsis.

Product Specs

Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
Form
Liquid
Lead Time
Made-to-order (14-16 weeks)
Synonyms
TPS07 antibody; At4g20200 antibody; F1C12.120 antibody; Putative terpenoid synthase 7 antibody; AtTPS07 antibody; EC 4.2.3.- antibody
Target Names
TPS07
Uniprot No.

Target Background

Database Links

KEGG: ath:AT4G20200

STRING: 3702.AT4G20200.1

UniGene: At.54427

Protein Families
Terpene synthase family, Tpsa subfamily
Subcellular Location
Cytoplasm.

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