TPT1P8 Antibody

Overview of TPT1P8 Antibody

The TPT1P8 antibody (STJ190305) is a rabbit polyclonal antibody developed against a synthesized peptide derived from the human TPT1P8 protein at amino acid residues 40–120 . It is primarily used for detecting TPT1P8 in research applications, including Western blot (WB) and ELISA .

Western Blot Performance

• Sample Tested: Lysates from HCT116 human colon carcinoma cells .

• Result: A distinct band at the expected molecular weight (~23 kDa) was observed, confirming specificity for TPT1P8 .

Immunocytochemistry (ICC)

• Localization: Cytoplasmic staining in HeLa cells, consistent with TPT1P8’s putative role in intracellular processes .

Biological Context of TPT1P8

TPT1P8 is a member of the translationally-controlled tumor protein (TCTP) family, which includes calcium-binding proteins involved in apoptosis regulation, cytokine secretion, and neurodegenerative diseases . While TPT1 (TCTP) is well-characterized, TPT1P8 remains less studied, with its exact function and interaction partners still under investigation.

Research Applications and Limitations

• Strengths:

  • High specificity for the 40–120 aa epitope of TPT1P8 .

  • Cross-reactivity with mouse and rat homologs enhances utility in preclinical models .

• Limitations:

  • No peer-reviewed studies directly linking TPT1P8 to disease mechanisms are available.

  • Limited commercial availability beyond research-grade reagents .

Comparative Analysis with Related Antibodies

Sources:

Future Directions

Further studies are needed to:

1. Elucidate TPT1P8’s role in cellular pathways (e.g., apoptosis, calcium signaling).

2. Validate its involvement in neurodegenerative or allergic diseases, as suggested by TCTP family studies .

3. Develop standardized assays for quantitative TPT1P8 detection in clinical samples.