TRAIP Antibody

TRAIP Antibody Overview

TRAIP antibodies are designed to target the 53.3 kDa TRAF-interacting protein, a 469-amino-acid protein localized in the nucleus and cytoplasm . Key features include:

• Synonyms: SCKL9, TRIP, RNF206, E3 ubiquitin-protein ligase TRAIP

• Protein Family: TRAIP/RNF206

• Post-Translational Modifications: Ubiquitination, sumoylation

• Orthologs: Conserved in mouse, rat, bovine, zebrafish, and primates

Research Applications

TRAIP antibodies are widely used to investigate:

• DNA Repair Mechanisms: TRAIP regulates DNA interstrand cross-link (ICL) repair by promoting CMG helicase convergence, critical for Fanconi anemia pathway activation .

• Replication-Transcription Conflicts: Depletion of TRAIP causes DNA damage at transcription start sites during S-phase, leading to G2 arrest and senescence .

• Genome Integrity: TRAIP binds PCNA and facilitates RPA-coated single-stranded DNA formation during replication stress, enhancing ATR-dependent checkpoint signaling .

DNA Damage Response

• TRAIP-deficient cells exhibit chromosomal instability and reduced survival under replication stress .

• In mitosis, TRAIP ubiquitylates CMG helicases to ensure replisome integrity .

Cell Cycle Regulation

• Rapid TRAIP degradation triggers G2 arrest and senescence, emphasizing its S-phase role in resolving replication-transcription collisions .

• TRAIP loss delays mitotic progression, independently contributing to DNA damage in subsequent cell cycles .

Disease Associations

• Cancer: TRAIP promotes proliferation in prostate cancer via TRAF2-PI3K-AKT pathway activation and enhances osteosarcoma invasion through IGFBP3/AKT signaling .

• Microcephalic Dwarfism: TRAIP mutations impair ICL repair, linking its dysfunction to developmental disorders .

Protocols and Validation

• Western Blot: Used to confirm TRAIP’s 53 kDa band in human and mouse lysates .

• Immunohistochemistry: Localizes TRAIP in nuclear and cytoplasmic compartments .

• Functional Studies: siRNA-mediated TRAIP knockdown reduces RPA foci formation under camptothecin-induced replication stress .