TRIM46 Antibody

Introduction

The TRIM46 antibody is a highly specific immunological reagent designed to detect the tripartite motif-containing protein 46 (TRIM46), a key regulator of neuronal polarity and axonal architecture. This antibody has gained prominence in neurobiology and oncology research due to its role in studying TRIM46’s involvement in both normal cellular processes and disease states. Below is a detailed analysis of its structure, applications, and research findings.

Structure and Function of TRIM46

TRIM46, also known as TRIFIC, belongs to the C-I TRIM family and localizes predominantly to the axon initial segment (AIS), a critical structure for neuronal signaling and polarization. Its primary functions include:

• Microtubule organization: Forms closely spaced parallel microtubule bundles essential for axonal transport and cargo delivery .

• Neuronal polarity: Establishes and maintains the separation between somatodendritic and axonal compartments .

• Cancer implications: Emerging evidence links TRIM46 to tumor progression, including lung adenocarcinoma (LUAD), where its overexpression correlates with poor prognosis .

Neurobiological Studies

• Axon Initial Segment (AIS) dynamics: TRIM46 antibodies have been used to study AIS microtubule organization via correlative light and electron microscopy. Depletion experiments revealed that TRIM46 is essential for cross-bridge formation between microtubules .

• Neuronal migration: Proteintech’s rabbit polyclonal antibody demonstrated TRIM46’s role in guiding neuronal migration during development .

Oncological Research

• Lung cancer (LUAD): Sysy’s guinea pig antibody was employed to show that TRIM46 overexpression promotes tumor growth, glycolysis, and cisplatin resistance by activating AKT signaling .

• Osteosarcoma: TRIM46’s ubiquitination of PPARα was visualized using Proteintech’s antibody, linking it to NF-κB activation and apoptosis inhibition .