TRM10 Antibody
Description
TRM10 Antibody Types and Targets
The TRM10 family includes three distinct homologs in humans: TRMT10A, TRMT10B, and TRMT10C. Each homolog exhibits unique substrate specificities and subcellular localizations, necessitating specialized antibodies for their detection.
2.1. Role in tRNA Modification
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TRMT10A is the primary enzyme responsible for m1G9 modifications in cytosolic tRNAs, critical for translation fidelity . Its deficiency correlates with impaired glucose metabolism and microcephaly .
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TRMT10B uniquely catalyzes m1A9 modifications in tRNA Asp, as demonstrated by HPLC-MS/MS analysis .
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TRMT10C is essential for mitochondrial tRNA 5'-end processing and respiration. Mutations in TRMT10C are linked to mitochondrial diseases .
2.2. Disease Implications
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Deletion of TRMT10A in yeast leads to hypersensitivity to 5-fluorouracil (5FU), a chemotherapeutic agent, due to reduced tRNA Trp levels .
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TRMT10C mutations impair mitochondrial translation, highlighting its role in energy metabolism .
2.3. Antibody Applications
TRM10 antibodies enable:
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Research has elucidated the mechanistic features of the atypical tRNA m1G9 SPOUT methyltransferase, Trm10. PMID: 28911116
- These findings establish Trm10 as a novel member of the SpoU-TrmD (SPOUT) MTase superfamily. PMID: 24081582
- In vitro methylation activity of Trm10 alone does not fully explain the observed modification pattern in vivo, suggesting the involvement of additional tRNA substrates for Trm10 m1G9 methyltransferase activity. PMID: 23793893
- Furthermore, research provides genetic evidence supporting a new role of Trm10p in translation termination efficiency. PMID: 21829385
KEGG: sce:YOL093W
STRING: 4932.YOL093W
