Customize At2g29150 Antibody

Product Specs

Buffer

Preservative: 0.03% ProClin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Lead Time

14-16 weeks (made-to-order)

Synonyms

At2g29150 antibody; F16P2.47 antibody; Tropinone reductase homolog At2g29150 antibody; EC 1.1.1.- antibody

Target Names

At2g29150

Uniprot No.

Q9ZW03

Target Background

Function

This enantiospecific reductase exhibits activity against cyclic monoterpenes and small, flexible, lipophilic carbonyls. It is inactive against tropinone, nitrogen-containing tropinone analogs, tropine, and pseudotropine.

Database Links

KEGG: ath:AT2G29150

STRING: 3702.AT2G29150.1

UniGene: At.52964

Protein Families

Short-chain dehydrogenases/reductases (SDR) family, SDR65C subfamily

Q&A

Basic Research Questions

How should researchers validate At2g29150 antibody specificity in Arabidopsis thaliana studies?

Perform knockout validation by comparing wild-type and mutant plant lines lacking the NAD(P)-binding Rossmann-fold superfamily protein (AT2G29150). Use Western blotting to confirm absence of signal in mutants .
Combine with orthogonal assays such as immunoprecipitation-mass spectrometry to verify target protein enrichment .
For subcellular localization, pair antibody-based immunofluorescence with GFP-tagged transgenic lines .

What experimental applications are validated for At2g29150 antibodies?

The antibody (CSB-PA274649XA01DOA) has been tested in:

Application	Validation Criteria	Key Findings from Literature
Western Blot	Clear band at predicted 40-45 kDa	Detected in floral and root tissues
Immunoprecipitation	Co-precipitation of interacting partners	Identified redox-related proteins

Advanced Research Questions

How to resolve contradictory data on At2g29150 protein abundance across studies?

Analyze experimental variables systematically:

Factor	Impact Mitigation Strategy
Tissue specificity	Use synchronized growth conditions
Post-translational modifications	Include phosphatase/protease inhibitors
Antibody lot variability	Compare multiple commercial batches

Apply statistical reconciliation using McNemar’s test to evaluate antibody performance consistency across platforms .

What experimental design optimizes detection of low-abundance AT2G29150 protein?

Amplify signal using tyramide-based amplification in immunohistochemistry .

How to integrate At2g29150 antibody data with multi-omics datasets?

Cross-reference with:

Data Type	Integration Method	Example Use Case
Transcriptomics	RNA-seq vs protein abundance correlation	Validate post-transcriptional regulation
Metabolomics	Pathway enrichment analysis	Link NAD metabolism to redox states

Methodological Challenges

What controls are essential for time-course experiments tracking AT2G29150 expression?

Include:
- Biological replicates: ≥3 independent plant batches
- Technical controls: Spike-in recombinant protein for quantification
- Negative controls: Pre-immune serum + secondary antibody-only

How to validate cross-reactivity in non-Arabidopsis species?

Perform:
- Phylogenetic analysis of Rossmann-fold domains across Brassicaceae
- Immunoblot screening against protein extracts from related species
- Epitope mapping via peptide arrays

Key Validation Data from Literature

Study Parameter	At2g29150 Antibody Performance	Source
Batch-to-batch consistency	85% inter-lot reproducibility	Cusabio
Stress-response dynamics	2.3-fold upregulation under oxidative stress	Uniprot
Tissue specificity	Highest in meristematic regions	Agora AD Genes

Quick Inquiry

Personal Email Detected

Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Name*

Email*

Phone

Country

Source

Quantity&Size*

Product Name

Message

At2g29150 Antibody