Trypsin Antibody

Definition and Structure

Trypsin antibodies target the trypsin enzyme (PRSS1), a 24 kDa protein produced in the pancreas as trypsinogen and activated in the gastrointestinal tract. These antibodies are classified by their specificity:

• Monoclonal antibodies: Engineered for high specificity, such as the TRYP11-236.7 clone (Thermo Fisher) .

• Polyclonal antibodies: Broader reactivity, often used in immunohistochemistry (e.g., Abcam's rabbit polyclonal) .

Key Features

• Gene aliases: PRSS1, TRP1, TRY1, TRYP1.

• UniProt ID: P07477 (human).

• Applications: ELISA, Western blot, immunoprecipitation (IP), and immunohistochemistry (IHC-P) .

2.1. Immunoassays

• ELISA: Detects trypsin levels in blood for screening cystic fibrosis (Thermo Fisher) .

• Western Blot: Confirms trypsin expression in tissues via antibodies like AF3586 (R&D Systems) .

2.2. Immunohematology

Trypsin antibodies resolve interference in antibody testing caused by daratumumab (DARA), a monoclonal therapy for multiple myeloma. A trypsin-based method eliminated DARA interference in 98.4% of samples, enabling accurate blood type screening .

2.3. Disease Diagnosis

• Cystic Fibrosis: High immunoreactive trypsin levels in blood are diagnostic markers .

• Pancreatic Disorders: Antibodies detect trypsin in pancreatic tissue to study chronic pancreatitis or cancer .

3.1. DARA Interference Resolution

A 2021 study demonstrated that trypsin treatment removed DARA interference in 60/61 (98.4%) antibody screens, maintaining detection of clinically significant alloantibodies .

3.2. Antibody Specificity

• Pancreas-Specific Staining: Antibodies like ab200997 (Abcam) show cytoplasmic staining in acinar cells .

• Cross-Reactivity: Some antibodies (e.g., AF3586) target multiple trypsin isoforms (PRSS1/2/3) .