twsg1a Antibody

Overview of TWSG1 and Its Antibodies

Twisted Gastrulation Protein 1 (TWSG1) is a secreted BMP-binding protein structurally related to BMP antagonists like Chordin and Noggin. It modulates BMP activity by directly binding to BMP ligands, influencing cell signaling pathways in development, tissue homeostasis, and immune regulation . TWSG1 antibodies are tools used to detect and study this protein in research settings, including applications like Western blotting (WB), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA).

Research Findings and Validation

• Bioss USA (bs-11606R): Validated for cross-reactivity in human, mouse, rat, dog, cow, sheep, and pig tissues. Demonstrated inhibition of BMP4 activity in biochemical assays .

• Antibodies Online (ABIN528066): Recognizes recombinant TWSG1 protein (26 kDa GST-tagged), with specificity confirmed via Western blotting .

• NovoPro Bioscience (116504): Detects a 45 kDa band in SH-SY5Y cells and mouse brain tissue. IHC validation shows staining in human kidney and mouse brain sections .

Applications in Research

Western Blotting:

• Bioss (1:300-5000): Detects TWSG1 in lysates from BMP-treated HuH-7 cells, confirming BMP-Smad signaling modulation .

• NovoPro (1:200-1000): Used to study TWSG1 expression in erythroblasts during maturation .

Immunohistochemistry:

• NovoPro (1:20-200): Identifies TWSG1 in paraffin-embedded mouse brain and human kidney tissues, highlighting its role in tissue-specific BMP regulation .

ELISA:

• Bioss (1:500-1000): Quantifies TWSG1 in conditioned media from BMP2-treated cells, showing dose-dependent inhibition .

Biological Context

TWSG1’s role in erythropoiesis was elucidated via studies in thalassemic mice, where its expression correlated with hepcidin regulation. Antibodies like those from NovoPro enabled detection of TWSG1 in murine spleen and erythroblast cultures, revealing its dynamic expression during maturation .