K11H3.3 Antibody

Research Findings

Mechanism

• Competes with ITS90.03 for overlapping epitopes but binds orthogonally, limiting gp41 interactions .

• Epitope occupancy depends on unoccupied PNGS sites (N247 and N254) on SIV Env .

Key Variant-Specific Antibodies

1. SNAP-Certified™ H3.3 Antibody

   • Specificity: Distinguishes H3.3 from H3.1 via serine substitution at amino acid 31 .

   • Applications: CUT&RUN chromatin profiling; no cross-reactivity with H3.1 or sensitivity to H3K27/K36 modifications .

   • Performance:

     • <20% cross-reactivity with other histones .

     • Genomic enrichment validated at 500k and 50k cell counts .

2. Anti-Histone H3.3 G34R Mutant Antibody (Clone RM240)

   • Specificity: Detects G34R mutation in glioblastoma tumors .

   • Validation:

     • Western blot and IHC confirmed specificity for mutant vs. wild-type H3.3 .

     • Clinically validated in FFPE glioblastoma samples .

Comparative Analysis

Critical Notes

• No sources link "K11H3.3" as a unified entity. The nomenclature may stem from confusion between K11 (virology) and H3.3 (epigenetics).

• Both antibody classes demonstrate high specificity but operate in distinct biological contexts.