ELI5 Antibody

Identification and Basic Characteristics

The ELI5 antibody (Product Code: CSB-PA851407XA01DOA) is a custom monoclonal antibody produced by Cusabio. Key identifiers include:

This antibody is part of a specialized collection of rare antibodies developed for plant research .

Target Protein: ELI5 in Arabidopsis thaliana

ELI5 (Early Light-Induced Protein 5) is a nuclear-encoded protein implicated in plant stress responses, particularly under high-light conditions. While the exact mechanistic role of ELI5 remains under investigation, homologs in Arabidopsis are associated with:

• Chloroplast function: Regulation of photosynthetic machinery during abiotic stress .

• DNA repair: Interaction with proteins involved in maintaining genomic stability .

The UniProt entry for Q8RY79 classifies ELI5 as a member of the DnaJ-like protein family, which typically functions as molecular chaperones .

Applications in Research

The ELI5 antibody is optimized for use in:

• Western Blot: Detecting ELI5 expression levels in Arabidopsis leaf extracts under stress conditions.

• Immunohistochemistry: Localizing ELI5 within chloroplasts and nuclei .

• ELISA: Quantifying ELI5 in plant tissue lysates .

Key Validation Criteria:

• Specificity: Validated using knockout (KO) Arabidopsis lines to confirm absence of cross-reactivity .

• Reproducibility: Batch-to-batch consistency ensured through recombinant antibody technology .

Antibody Development Trends:

• Recombinant Antibodies: The ELI5 antibody aligns with industry shifts toward recombinant antibodies, which show higher specificity and reproducibility compared to polyclonal counterparts .

• High-Throughput Screening: Platforms like LIBRA-seq enable rapid identification of antibodies targeting specific epitopes, as seen in viral research .

Challenges in Plant Antibody Research:

• Low Commercial Availability: Only ~12% of plant proteins have commercially available antibodies, making tools like the ELI5 antibody critical for niche studies .

• Epitope Conservation: Plant proteins often share epitopes across species, necessitating rigorous validation to avoid off-target binding .

Availability and Usage Notes

The ELI5 antibody is available through Cusabio’s Custom Antibodies project, which focuses on rare targets in plant biology . Researchers are advised to:

• Use KO controls to validate specificity in Arabidopsis experiments .

• Optimize protocols for chloroplast lysis when working with photosynthetic tissues .

Future Directions

While no direct studies on ELI5’s functional role were identified in the reviewed literature, its homology to stress-response proteins suggests potential research avenues:

• Investigating ELI5’s role in oxidative stress mitigation.

• Exploring interactions with DNA repair pathways using co-immunoprecipitation (Co-IP) .