Customize At1g62220 Antibody

Description

Gene Overview

AT1G62220 is annotated as a transmembrane protein in the Arabidopsis thaliana genome. Its exact biological role remains understudied, with no direct functional studies identified in peer-reviewed literature.

Attribute	Details
Gene ID	AT1G62220
Organism	Arabidopsis thaliana
Protein Class	Transmembrane protein
Computational Annotation	Described as a transmembrane protein by TAIR (The Arabidopsis Information Resource) .

Antibody Availability and Research Gaps

No commercially available or peer-reviewed antibodies targeting AT1G62220 were identified across databases or publications. Key observations:

Uncharacterized Target: AT1G62220 lacks functional studies, making antibody development or validation unlikely.
Homology Considerations: No homologs in well-studied organisms (e.g., humans) were reported, limiting cross-species antibody applications.

Related Arabidopsis Seed Coat Proteins

While AT1G62220 itself is uncharacterized, research on Arabidopsis seed coat proteins provides context for transmembrane protein families in this organism. For example:

Accession No.	Gene	Protein Function	Localization
AT1G62080	TBA3	Unknown	Seed coat mucilage
AT1G62000	TBA1	Unknown	Seed coat mucilage
AT3G04170	RmlC-like	Oxidoreductase activity	Adherent mucilage layer

These proteins are secreted during seed coat differentiation but share no homology with AT1G62220 .

Challenges in Antibody Validation

Studies on other plant antibodies highlight broader challenges:

Specificity Issues: Commercial antibodies often fail validation in plant systems due to cross-reactivity (e.g., non-specific binding to unrelated epitopes) .
Structural Complexity: Transmembrane proteins like AT1G62220 require specialized antibodies for extracellular vs. intracellular domain targeting, complicating development .

Future Directions

Proposed steps to advance AT1G62220 antibody research:

Functional Characterization: Clarify the protein’s role in Arabidopsis physiology (e.g., via knockout mutants).
Epitope Design: Prioritize extracellular domains for antibody development to avoid transmembrane region interference.
Collaborative Efforts: Leverage plant biology consortia (e.g., TAIR, ABRC) for resource sharing.

Product Specs

Buffer

Preservative: 0.03% Proclin 300
Composition: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4

Form

Liquid

Lead Time

Made-to-order (14-16 weeks)

Synonyms

At1g62220 antibody; F19K23.15 antibody; UPF0540 protein At1g62220 antibody

Target Names

At1g62220

Uniprot No.

O04587

Q&A

Here’s a structured collection of FAQs for researchers working with At1g62220 Antibody (Uniprot: Q6DYE5), tailored to academic research scenarios and synthesized from multidisciplinary sources:

Advanced Research Questions

Resolving contradictions in At1g62220 subcellular localization studies
- Approach:
  - Compare results across methods (e.g., confocal microscopy vs. cell fractionation + Western blot).
  - Account for fixation artifacts by testing multiple protocols (e.g., paraformaldehyde vs. methanol).
  - Validate with orthogonal techniques like in situ hybridization or GFP-tagged constructs .
Epitope mapping for At1g62220 Antibody
- Steps:
  1. Generate truncation mutants of At1g62220 (e.g., N-terminal vs. C-terminal deletions).
  2. Test antibody binding via dot blot or SPR.
  3. Use structural prediction tools (e.g., AlphaFold) to model antibody-antigen interfaces .
Quantifying At1g62220 expression under stress conditions
- Workflow:
  - Combine qRT-PCR (transcript levels) with antibody-based ELISA (protein levels).
  - Normalize data to housekeeping genes/proteins (e.g., ACTIN2 or RUBISCO).
  - Account for post-translational modifications via 2D gel electrophoresis .

Table 1: Key Parameters for At1g62220 Antibody Validation

Parameter	Method Used	Result
Specificity	Western blot (KO vs. WT)	No band in KO lines
Cross-reactivity	ELISA (UGT71 family)	<5% binding to UGT71B6/UGT71C1
Optimal IHC dilution	Titration (0.625 µg/mL)	Clear signal in vascular tissues

Table 2: Troubleshooting Common Issues

Issue	Solution
High background in IHC	Increase blocking time; reduce antibody concentration
Inconsistent Western blot bands	Use fresh protease inhibitors; validate lysate pH
Weak signal in ELISA	Optimize antigen coating density (1–5 µg/mL)

Methodological Recommendations

For multiplex assays, balance antibody concentrations to prevent signal dominance (e.g., limit high-abundance targets to ≤1 µg/mL) .
In longitudinal studies, aliquot antibodies to minimize freeze-thaw cycles and track lot-to-lot variability .
Use machine learning tools (e.g., CellProfiler) for high-throughput image analysis of antibody staining patterns .

Quick Inquiry

Personal Email Detected

Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Name*

Email*

Phone

Country

Source

Quantity&Size*

Product Name

Message

At1g62220 Antibody