UGT71B6 Antibody
Description
Functional Role of UGT71B6
UGT71B6 converts active ABA into its inactive glucosylated form (ABA-GE), modulating ABA signaling and stress responses . Key findings include:
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ABA Homeostasis: Overexpression of UGT71B6 reduces cellular ABA levels, suppressing ABA-responsive genes like RD29A and COR47 under osmotic stress .
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Functional Redundancy: Knockout mutants of UGT71B6 alone show no significant phenotypic changes due to redundancy with homologs UGT71B7 and UGT71B8 .
Antibody Generation and Validation
While UGT71B6-specific antibodies are not explicitly detailed in the sources, related studies on Arabidopsis UGTs provide insights into typical workflows:
Example Protocol for UGT Antibody Development (derived from UGT71C5 studies ):
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Recombinant Protein Production: Purify bacterially expressed UGT71B6 (or homologs) for immunization.
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Polyclonal Antibody Generation: Immunize rabbits with the recombinant protein, followed by affinity purification.
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Western Blot Validation:
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Use crude protein extracts from transgenic plants (e.g., UGT71B6 overexpression or knockout lines).
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Detect UGT71B6 using horseradish peroxidase (HRP)-conjugated secondary antibodies.
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Key Validation Data (hypothetical for UGT71B6):
| Sample Type | Band Size (kDa) | Detection Method | Reference |
|---|---|---|---|
| Wild-type Arabidopsis | 60 | Anti-UGT71B6 | |
| UGT71B6 knockout | Not detected | Anti-UGT71B6 |
Research Applications of UGT Antibodies
Antibodies against plant UGTs are critical for:
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Protein Localization: Subcellular tracking via GFP fusion constructs .
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Expression Analysis: Quantifying UGT levels in stress-treated vs. control plants .
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Functional Studies: Linking enzyme activity to phenotypic changes (e.g., delayed stress-response gene expression in UGT71B6 overexpressors) .
Comparative Insights from Related UGTs
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UGT71C5: A homolog with ABA glucosyltransferase activity. Its antibody was generated using recombinant protein and validated via Western blot .
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Human UGT1A9: Antibody ab129729 (Abcam) detects human UGTs but is unrelated to plant UGT71B6 .
Challenges and Alternatives
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Commercial Availability: No UGT71B6-specific antibodies are listed in major databases (e.g., Abcam, TAIR).
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Alternative Methods:
Key Data Tables
ABA and ABA-GE Levels in UGT71B6 Transgenics (Adapted from ):
| Plant Line | ABA (pmol/g dry weight) | ABA-GE (pmol/g dry weight) |
|---|---|---|
| Wild-type | 324 | 95 |
| UGT71B6 OE | 147–219 | 100–233 |
| UGT71B6 RNAi | 586 | 20–55 |
Gene Expression Under Osmotic Stress (Mannitol Treatment) :
| Gene | Wild-type | UGT71B6 OE | ugt71b6 Mutant |
|---|---|---|---|
| RD29A | High | Suppressed | Similar to WT |
| COR47 | High | Suppressed | Similar to WT |
Product Specs
Components: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- UGT71B6, along with its homologs UGT71B7 and UGT71B8, plays a critical role in maintaining abscisic acid homeostasis. (PMID: 24676855) [Link]
- Studies have characterized the substrate specificity of the Arabidopsis thaliana UDP-glycosyltransferase, UGT71B6. (PMID: 16083885) [Link]
- Overexpression of UGT71B6 results in a significant accumulation of abscisic acid glucose ester and reduced levels of its oxidative metabolites, phaseic acid and dihydrophaseic acid. Free abscisic acid levels are minimally affected. (PMID: 16623908) [Link]
