rfl-1 Antibody
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Product Specs
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Made-to-order (14-16 weeks)
Synonyms
rfl-1 antibody; uba-3 antibody; F11H8.1 antibody; NEDD8-activating enzyme E1 catalytic subunit antibody; EC 6.2.1.- antibody; Ectopic membrane ruffles in embryo protein 1 antibody; Ubiquitin-activating enzyme 3 homolog antibody
Target Names
rfl-1
Uniprot No.
Target Background
Function
The rfl-1 antibody targets the catalytic subunit of the dimeric rfl-1 (uba-3)-ula-1 E1 enzyme. This enzyme plays a crucial role in NEDD8 activation, a process essential for cellular function. Specifically, E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, then linking this residue to the side chain of the catalytic cysteine. This results in the formation of a NEDD8-uba-3 thioester and free AMP. Finally, E1 transfers NEDD8 to the catalytic cysteine of ubc-12. This enzymatic activity is vital for processes such as cytokinesis and mitotic spindle orientation, particularly during early embryogenesis.
Gene References Into Functions
- Depletion of the strongest specific suppressors rescued the early embryonic cell division defects in rfl-1 mutants. Interestingly, depletion of the two strongest specific enhancers did not affect the early embryonic cell division defects. PMID: 19528325
Database Links
Protein Families
Ubiquitin-activating E1 family, UBA3 subfamily
Subcellular Location
Nucleus. Cytoplasm.
Tissue Specificity
Expressed in intestine, vulva epithelium and head and tail neurons.
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