uba-5 Antibody
Description
Research Applications
UBA5 antibodies are widely used in:
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Western Blot (WB): Detects UBA5 at ~45 kDa in lysates from HeLa, HEK-293, Jurkat, and cancer cell lines .
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Immunohistochemistry (IHC): Localizes UBA5 in breast cancer, colorectal adenocarcinoma, and other tissues .
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Immunoprecipitation (IP): Identifies UBA5 interaction partners (e.g., UFM1, UFC1) .
Functional Insights from Antibody Studies
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Role in Cancer: Overexpression of UBA5 correlates with poor prognosis in breast, lung, and pancreatic cancers. Antibody-based assays reveal elevated UBA5 levels in tumor lysates .
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Neurological Disorders: UBA5 knockdown models show impaired erythroid differentiation and megakaryopoiesis, rescued by transgenic UBA5 expression .
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Therapeutic Targeting: Inhibitors like DKM disrupt UBA5-UFM1 binding in FP assays (Z’ = 0.5, CV = 8.2%), validated using UBA5 antibodies .
Clinical Relevance
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Biomarker Potential: UBA5 antibodies identify overexpression in glioblastoma and renal cancer tissues .
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Therapeutic Development: FP and AMP-Glo™ assays using UBA5 antibodies screen inhibitors for neurodegenerative and oncological applications .
Western Blot (Boster Bio A06385-2)
| Sample | Band Intensity | Observed MW |
|---|---|---|
| Human HeLa lysate | High | 45 kDa |
| Mouse pancreas lysate | Moderate | 45 kDa |
| Rat pancreas lysate | Low | 45 kDa |
IHC in Human Tissues
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Clinical, biochemical, and experimental findings support the conclusion that UBA5 mutations are a key cause of early-onset encephalopathies resulting from abnormal protein ufmylation. PMID: 27545681
KEGG: cel:CELE_T03F1.1
STRING: 6239.T03F1.1.1
