UBASH3B Antibody
Description
Prognostic Biomarker Identification
UBASH3B antibody enabled the discovery of UBASH3B as a poor prognostic marker in prostate cancer (PCa):
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Upregulation: mRNA and protein levels were significantly elevated in PCa compared to benign tissues (p < 0.05) .
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Survival Correlation: High UBASH3B expression correlated with reduced 5-year survival rates (HR = 2.1, p = 0.003) .
Tumor Microenvironment Analysis
The antibody facilitated immune cell infiltration studies in PCa using CIBERSORT and TIMER databases:
| Immune Cell Type | Correlation with UBASH3B | p-Value |
|---|---|---|
| Activated CD4+ memory T cells | Positive | 0.0274 |
| M2 macrophages | Positive | 0.0001 |
| Resting dendritic cells | Positive | <0.0001 |
| Neutrophils | Positive | 0.0499 |
| Source: |
Molecular Pathways
UBASH3B antibody-based studies revealed its involvement in:
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EGFR Regulation: Dephosphorylation of CBL ubiquitin ligase, stabilizing EGFR in triple-negative breast cancer .
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Immune Signaling: Interaction with LCP2 (lymphocyte cytosolic protein 2) in PCa, influencing T-cell receptor pathways .
ceRNA Network Construction
UBASH3B antibody helped validate its role in competing endogenous RNA (ceRNA) networks:
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Associated miRNAs: miR-200a-3p, miR-455-5p (downregulated in high UBASH3B PCa) .
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lncRNA Partners: MIAT, LINC01297 (positively correlated with UBASH3B expression) .
Therapeutic Targeting
UBASH3B’s phosphatase activity makes it a druggable target:
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Inhibition: Reduces EGF-induced invasion in PC3 and DU145 prostate cancer cells (p < 0.01) .
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Immune Modulation: Linked to 11 tumor-infiltrating immune cell types, suggesting combinational immunotherapy potential .
Limitations and Future Directions
Product Specs
Target Background
- Studies indicate that Sts1 (suppressor of T-cell receptor signaling-1) negatively regulates TCR (T-cell receptor) signaling in activated T-lymphocytes by dephosphorylating ZAP70. PMID: 24256567
- Research shows that the interaction between STS-1 and ShcA is regulated in response to EGF receptor activation. PMID: 28690151
- A study reveals a role of CBL in restricting myeloid proliferation of human AML1-ETO-induced leukemia, and identifies UBASH3B as a potential target for pharmaceutical intervention. PMID: 26449661
- Targeting Aurora B to microtubules by UBASH3B is essential for the timing and fidelity of chromosome segregation in human cells. PMID: 26766443
- Results demonstrate that TULA-2 is a target of miR-148a-3p. PMID: 26516227
- This recovery involves the phosphorylation of p70 independent of mTOR. PMID: 25271148
- Results demonstrate the importance of STS-1 in regulating IFN-alpha-induced autophagy in B cells. PMID: 25959715
- UBASH3B is a functional target of anti-invasive microRNA200a that is down-regulated in triple negative breast cancer. Importantly, the oncogenic potential of UBASH3B is dependent on its tyrosine phosphatase activity. PMID: 23784775
- Ablation of TULA-2 resulted in hyperphosphorylation of Syk and its downstream effector phospholipase C-gamma2 as well as enhanced GPVI-mediated platelet functional responses. PMID: 20585042
- hsp70 inhibits apoptosis upstream and downstream of the mitochondria and is a promising therapeutic target for reversing drug-resistance in chronic myeloid leukemia-blast crisis and acute myeloid leukemia cells. PMID: 15388581
